The frequency characteristics analysis of series photodetector frequency circuit system based on circuit parameters of quartz crystal and its application for bacterial DNA identification

The circuit parameters of quartz crystal were employed for frequency sensitivity analysis of series photodetector frequency circuit system. The influence of circuit parameters of quartz crystal on the oscillation frequency and response sensitivity were theoretically derived and experimentally verifi...

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Bibliographic Details
Published in:Optik (Stuttgart) 2014-03, Vol.125 (6), p.1715-1722
Main Authors: Hsu, Yuh-Ming, Chang, Chung-Cheng
Format: Article
Language:English
Subjects:
Aeromonas hydrophila
Bacteria
Circuits
Deoxyribonucleic acid
DNA probe
Dyes
Fluorescence
Fluorescence dye concentration
Hybridization
Photodetector
Photodetectors
Quartz crystal
Quartz crystals
Sensitivity analysis
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Summary:The circuit parameters of quartz crystal were employed for frequency sensitivity analysis of series photodetector frequency circuit system. The influence of circuit parameters of quartz crystal on the oscillation frequency and response sensitivity were theoretically derived and experimentally verified. On the basis of optimal circuit parameters, the DNA probe detection limit 2pmol/L can be measured by 49.4MHz sensor system. In comparison with the conventional fluorescence technique, the frequency method showed that the detection limits of DNA probe AH642 with Cy5 fluorescence dye and DNA probe VA180 with Cy5 fluorescence dye were lower than the conventional fluorescence technique by 2–3 orders; meanwhile, through the feature of probe uniqueness, Aeromonas hydrophila DNA and fluorescence probe AH642-Cy5 can be successfully judged for hybridization reaction. Moreover, Vibro alginolyticus DNA and fluorescence probe VA180-Cy5 can be successfully judged for hybridization reaction.
ISSN:0030-4026
1618-1336
DOI:10.1016/j.ijleo.2013.09.061