Multifunctional Fluorescent Probe for Sequential Detections of Glutathione and Caspase‑3 in Vitro and in Cells

Herein, we report a new “On–On” strategy based on the assembly and disassembly of fluorescein isothiocyanate nanoparticles (FITC-NPs) for sequential detections of glutathione (GSH) and caspase-3 (Casp3) with a multifunctional fluorescent probe 1. Theoretical investigations revealed the underlying me...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2013-07, Vol.85 (13), p.6203-6207
Main Authors: Huang, Rui, Wang, Xijun, Wang, Dingli, Liu, Fang, Mei, Bin, Tang, Anming, Jiang, Jun, Liang, Gaolin
Format: Article
Language:English
Subjects:
Analytical chemistry
Apoptosis
Assembly
Biosensing Techniques - methods
Caspase 3 - analysis
Cell Line, Tumor
Chemical compounds
Disassembly
Dismantling
Fluorescein
Fluorescein-5-isothiocyanate - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Glutathione
Glutathione - analysis
Humans
Nanoparticles
Nanoparticles - chemistry
Peptides
Proteins
Spectrometry, Fluorescence - methods
Strategy
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Summary:Herein, we report a new “On–On” strategy based on the assembly and disassembly of fluorescein isothiocyanate nanoparticles (FITC-NPs) for sequential detections of glutathione (GSH) and caspase-3 (Casp3) with a multifunctional fluorescent probe 1. Theoretical investigations revealed the underlying mechanism that satisfactorily explained experimental results of such consecutive enhancements of fluorescence. Using this probe, we also successfully imaged the Casp3 activity in apoptotic cells.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac4014012