Real-Time NMR Monitoring of Protein-Folding Kinetics by a Recycle Flow System for Temperature Jump

An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by a temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by ra...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2013-10, Vol.85 (20), p.9439-9443
Main Authors: Yamasaki, Kazuhiko, Obara, Yuji, Hasegawa, Manabu, Tanaka, Hideki, Yamasaki, Tomoko, Wakuda, Tsuyoshi, Okada, Michiya, Kohzuma, Takamitsu
Format: Article
Language:English
Subjects:
Animals
Cattle
Cooling systems
Entropy
Heating
Instability
Kinetics
Magnetic Resonance Spectroscopy - methods
Monitoring
NMR
Nuclear magnetic resonance
Principal Component Analysis
Principal components analysis
Protein Folding
Rate constants
Reaction kinetics
Real time
Ribonuclease, Pancreatic - chemistry
Symbols
Temperature
Temperature effects
Time Factors
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Summary:An NMR method was developed that allows for real-time monitoring of reactions (on the order of seconds) induced by a temperature jump. In a recycle flow system, heating and cooling baths were integrated, with the latter inside the NMR probe. A refolding reaction of ribonuclease A was triggered by rapid cooling and monitored by a series of NMR measurements over 12 s. Data were processed by principal component analysis, in which a factor related to the structural change with an exponential rate constant of 0.2–0.7 s–1 was successfully separated from factors related to baseline instability and/or noise. Temperature dependency of the rate constant revealed the entropy-driven formation of the transition state of the refolding reaction.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac401579e