Assessment of Local Tobacco Consumption by Liquid Chromatography–Tandem Mass Spectrometry Sewage Analysis of Nicotine and Its Metabolites, Cotinine and trans-3′-Hydroxycotinine, after Enzymatic Deconjugation

• Published in: Analytical chemistry (Washington) 2014-10, Vol.86 (20), p.10274-10281
• Main Authors: Rodríguez-Álvarez, Tania, Rodil, Rosario, Rico, María, Cela, Rafael, Quintana, José Benito
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Assessments; Biomarkers; Chemistry Techniques, Analytical - methods; Chromatography; Chromatography, Liquid; Consumption; Cotinine - analogs & derivatives; Cotinine - analysis; Glucose; Humans; Mass spectrometry; Nicotine; Nicotine - analysis; Nicotine - metabolism; Population Surveillance; Samples; Sewage; Sewage - chemistry; Spain - epidemiology; Statistical methods; Tandem Mass Spectrometry; Tobacco; Tobacco Use - epidemiology
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac503330c

Cotinine (COT), trans-3′-hydroxycotinine (OH-COT), cotinine-N-β-glucuronide (COT-GLUC), and trans-3′-hydroxycotinine-O-β-glucuronide (OH-COT-GLUC) are excreted in urine following the intake of nicotine (NIC), and, as such, they have been detected in sewage. Thus, they also constitute convenient biomarkers for NIC tracing through the sewage epidemiology approach at the local scale. Such estimation requires granting a good stability of the target biomarkers in sewage. However, it was found that glucuronides are not stable, particularly in the case of OH-COT-GLUC, which could render variable concentrations of COT, OH-COT, and their glucuronides, depending on sampling and storage time or temperature. Thus, an enzymatic deconjugation with β-glucuronidase was optimized. With the optimized method, after enzymatic deglucuronization, the limits of quantification obtained were in the range of 0.2–1 μg L–1, relative standard deviations were