Uptake and accumulation of B-group vitamers in Saccharomyces cerevisiae in ethanol-stat fed-batch culture

The uptake and accumulation of the B-group vitamins thiamine, riboflavin, nicotinamide, pantothenic acid and pyridoxine in Saccharomyces cerevisiae was studied by gradually increasing the specific dosage of vitamins in an ethanol-stat fed-batch culture. Thiamine, nicotinamide, pantothenic acid, and...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2014-09, Vol.30 (9), p.2351-2359
Main Authors: Paalme, T, Kevvai, K, Vilbaste, A, Hälvin, K, Nisamedtinov, I
Format: Article
Language:English
Subjects:
Accumulation
Acids
Agricultural biotechnology
Applied Microbiology
Assimilation
Batch Cell Culture Techniques
Biochemistry
Biomedical and Life Sciences
Biotechnology
Culture
culture media
Culture Media - chemistry
Dosage
Environmental Engineering/Biotechnology
Enzymes
Ethanol
Ethanol - metabolism
Grain
Kinases
Life Sciences
Metabolism
Microbiology
Nicotinamide
Original Paper
pantothenic acid
Pyridoxine
Riboflavin
Saccharomyces cerevisiae
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Studies
thiamin
Thiamine
Uptakes
Vitamin B
Vitamin B Complex - metabolism
Vitamins
Yeast
Yeasts
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Summary:The uptake and accumulation of the B-group vitamins thiamine, riboflavin, nicotinamide, pantothenic acid and pyridoxine in Saccharomyces cerevisiae was studied by gradually increasing the specific dosage of vitamins in an ethanol-stat fed-batch culture. Thiamine, nicotinamide, pantothenic acid, and pyridoxine were almost completely taken up at low vitamin dosages. Thiamine was determined to be the major accumulating form of vitamin B₁ while most of the assimilated nicotinamide and pantothenic acid accumulated in cofactor forms. Despite the obvious uptake of pyridoxine, accumulation of B₆ vitamers was not observed. In contrast with the other vitamins studied, riboflavin began accumulating in the culture medium immediately after vitamin addition was initiated. By the end of the experiment, the apparent uptake of all vitamins exceeded their accumulation in the cells. Variations in the growth rate of yeast at different vitamin dosages demonstrate the importance of balancing the vitamins in the media during cultivation.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-014-1660-x