Effects of different selenium levels on growth and regulation of laccase and versatile peroxidase in white-rot fungus, Pleurotus eryngii

• Published in: World journal of microbiology & biotechnology 2014-07, Vol.30 (7), p.2101-2109
• Main Authors: Kim, Yong Hyun, Lee, Hyoun-Su, Kwon, Hyun-Jung, Patnaik, Bharat Bhusan, Nam, Kung-Woo, Han, Yeon Soo, Bang, In-Seok, Han, Man-Deuk
• Format: Article
• Language: English
• Subjects: Applied Microbiology; Biochemistry; Biodegradation; Biomass; Biomedical and Life Sciences; Biotechnology; Control; culture media; Environmental Engineering/Biotechnology; enzyme activity; Enzymes; Fungi; Gene expression; gene expression regulation; hyphae; Inhibition; Laccase; Laccase - metabolism; Life Sciences; Lignin; messenger RNA; microbial biomass; microbial growth; Microbiology; mycelium; Original Paper; Peroxidase; Peroxidase - metabolism; Pleurotus - drug effects; Pleurotus - enzymology; Pleurotus eryngii; reducing sugars; Rhus; Scanning electron microscopy; Selenium; Selenium - pharmacology; Sodium; sodium selenite; Studies; Toxicity; Toxicodendron vernicifluum; Trace elements; white-rot fungi
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-014-1636-x

This study was conducted to evaluate the physiological effects of different selenium (Se) levels on the growth of white-rot fungus, Pleurotus eryngii, with special reference to the regulation of ligninolytic enzymes such as laccase and versatile peroxidase. The fungus was grown in medium supplemented with 1, 10, 100, 1,000 and 10,000 μM of sodium selenite. Mycelial growth was stronger at lower Se levels, but declined significantly at higher concentrations of 1,000 and 10,000 μM, highlighting its association in mediating toxic responses. Inhibition of fungal growth was accompanied with dense and entangled hyphae taking the shape of irregular short strips. Additionally, hyphal swellings and septation were noticed which lead to a reduction in the advancement of the mycelium. Along with the inhibition of fungal biomass, the reducing sugar and protein concentrations increased to about 30.2 and 3.5 mg/ml respectively in the growth medium. Additionally, the laccase gene expression showed a twofold upregulation at higher levels of Se, although the activity of the enzyme was compromised with an inverse relationship with increased gene transcripts. The versatile peroxidase transcript showed a complete downregulation at 10,000 μM after an upregulation at lower levels of Se. We also confirmed the direct relationship of different Se levels on laccase activity of Rhus vernicifera that showed similar behavior to the fungal laccase. The results of the present study suggest that Se supplementation regulates mRNA levels of laccase and versatile peroxidase depending on exposure and may play a role in the toxicity associated with Se.