Drug delivery and tissue engineering applications of biocompatible pectin–chitin/nano CaCO3 composite scaffolds

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-06, Vol.106, p.109-116
• Main Authors: Kumar, P.T. Sudheesh, Ramya, C., Jayakumar, R., Nair, Shanti kumar V., Lakshmanan, Vinoth-Kumar
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials; biomineralization; Calcium carbonate; Calcium Carbonate - chemistry; Cell Line; Chitin; Chitin - chemistry; colloids; Drug delivery; Drug Delivery Systems; drugs; fibroblasts; Fourier transform infrared spectroscopy; freeze drying; Humans; Mice; Microscopy, Electron, Scanning; Nanocomposite Scaffolds; Nanocomposites; Nanomaterials; Nanostructure; Pectin; pectins; Pectins - chemistry; Scaffolds; scanning electron microscopes; scanning electron microscopy; Spectroscopy, Fourier Transform Infrared; Swelling; Tissue Engineering; Tissue Scaffolds; toxicity; X-Ray Diffraction
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2013.01.048

[Display omitted] ► Nanocomposite scaffold comprised of pectin–chitin/nano CaCO3 has been developed and characterized. ► The developed composite scaffold showed controlled swelling and degradation. ► Nanocomposite scaffold was cytocompatible and cells were started attachment and proliferation. ► The drug delivery using the nanocomposite scaffolds was examined using a bisphosphonate called Fosamax. ► The nanocomposite scaffold can be a better candidate for bone regeneration and drug delivery. In this work, we have developed a nanocomposite scaffold using a mixture of pectin, chitin and nano CaCO3 using the technique of lyophilization, with an intended use towards biomedical applications such as tissue engineering and drug delivery. The prepared composite scaffold was characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). In addition, swelling, degradation and biomineralization capability of the composite scaffold was evaluated. The developed composite scaffold showed controlled swelling and degradation in comparison with the control scaffold. Cytocompatibility evaluation of the scaffold was tested on NIH3T3, L929 and human dermal fibroblast (HDF) cells, showed negligible toxicity towards cells. Cell attachment and proliferation studies were also conducted using these cells, which showed that cells attached onto the scaffolds and started to proliferate after 48h of incubation. Further, drug delivery through the scaffold was examined using a bisphosphonate called Fosamax. These results suggest that the developed composite scaffold possess the essential requisites for their application in the fields of tissue engineering and drug delivery.