Dependence of Filopodia Morphology and the Separation Efficiency of Primary CD4+ T‑Lymphocytes on Nanopillars

Despite significant improvement in separation efficiency using nanostructure-based platforms, the mechanism underlying the high efficiency of rare cell capture remains elusive. Here we report on the first mechanistic study by developing highly controlled nanostructures to investigate cell surface na...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2014-06, Vol.86 (11), p.5330-5337
Main Authors: Kim, Gil-Sung, Kim, Dong-Joo, Hyung, Jung-Hwan, Lee, Myung Kyu, Lee, Sang-Kwon
Format: Article
Language:English
Subjects:
Adhesion
Animals
Arrays
Binding sites
CD4-Positive T-Lymphocytes - chemistry
CD4-Positive T-Lymphocytes - ultrastructure
Cell adhesion & migration
Contact
Lymphocytes
Membrane Proteins - chemistry
Mice
Mice, Inbred C57BL
Morphology
Nanostructure
Nanostructured materials
Nanostructures
Pseudopodia - chemistry
Pseudopodia - ultrastructure
Quartz
Rendering
Separation
Spleen - cytology
T cell receptors
Three dimensional
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Summary:Despite significant improvement in separation efficiency using nanostructure-based platforms, the mechanism underlying the high efficiency of rare cell capture remains elusive. Here we report on the first mechanistic study by developing highly controlled nanostructures to investigate cell surface nanomorphology to better understand the cellular response of CD4+ T-lymphocytes in contact with nanostructured surfaces and to elucidate key mechanisms for enhancing separation efficiency. Our results showed that actin-rich filopodia protruded from T-cells in the early stage of cell capture (
ISSN:0003-2700
1520-6882
DOI:10.1021/ac5001916