Low-Interference Washing-Free Electrochemical Immunosensor Using Glycerol-3-phosphate Dehydrogenase as an Enzyme Label

In washing-free electrochemical detection, various redox and reactive species cause significant interference. To minimize this interference, we report a washing-free electrochemical immunosensor using flavin adenine dinucleotide (FAD)-dependent glycerol-3-phosphate dehydrogenase (GPDH) and glycerol-...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2015-04, Vol.87 (7), p.3574-3578
Main Authors: Dutta, Gorachand, Park, Seonhwa, Singh, Amardeep, Seo, Jeongwook, Kim, Sinyoung, Yang, Haesik
Format: Article
Language:English
Subjects:
Analytical chemistry
Antibodies, Immobilized - chemistry
Biosensing Techniques - methods
Chemistry
Electrochemical Techniques - methods
Electrodes
Electrons
Enzymes
Glycerol
Glycerolphosphate Dehydrogenase
Humans
Immunoenzyme Techniques - methods
Immunosensors
Interference
Labels
Limit of Detection
Oxidation
Sensors
Silver
Symbols
Tin Compounds - chemistry
Troponin I - analysis
Troponin I - blood
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Summary:In washing-free electrochemical detection, various redox and reactive species cause significant interference. To minimize this interference, we report a washing-free electrochemical immunosensor using flavin adenine dinucleotide (FAD)-dependent glycerol-3-phosphate dehydrogenase (GPDH) and glycerol-3-phosphate (GP) as an enzyme label and its substrate, respectively, because the reaction of FAD-dependent dehydrogenases with dissolved O2 is slow and the level of GP preexisting in blood is low (
ISSN:0003-2700
1520-6882
DOI:10.1021/ac504485a