Variation in recombinant protein expression levels among clones of lepidopteran cell populations

• Published in: Enzyme and microbial technology 1995, Vol.17 (2), p.168-174
• Main Authors: Pasumarthy, Murali K., Murhammer, David W.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; clonal isolates; Escherichia coli; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Insect cells; Methods. Procedures. Technologies; Modification of gene expression level; recombinant protein expression; Spodoptera frugiperda
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/0141-0229(94)00018-M

Clones isolated from the heterogenous Spodoptera frugiperda IPLB-Sf-21-AE and Mamestra brassiace IZD-Mb-O503 insect cell populations have been compared in regards to their synthesis of recombinant Escherichia coli β-galactosidase (β-gal), human tissue plasminogen activator (t-PA), and human kidney renin. Significant differences were found among the clones in their expression levels of these proteins. In addition, the expression levels oft-PA and renin were considerably less than that of β-gal. Clone BC5 was the best producer of t-PA and renin among the Mb-O503 clones, whereas clone BB5 was the best producer of β-gal. The Sf-9 cell line and clone 2 were the best producers of t-PA and renin, respectively, among the Sf-21 clones. The Mb-O503 clones generally were better producers of t-PA than the Sf-21 clones, with the BC5 clone producing twice the levels of any other Mb-O503 or Sf-21 clone. The Sf-21 clones were generally better renin producers; the Mb-O503 clones were generally found to have β-gal expression levels superior to those reported previously for the Sf-21 clones.