Expression of Leptin and Visfatin in Gingival Tissues of Chronic Periodontitis With and Without Type 2 Diabetes Mellitus: A Study Using Enzyme‐Linked Immunosorbent Assay and Real‐Time Polymerase Chain Reaction

Background: The aim of this study is to investigate the protein and gene expression of leptin and visfatin in gingival tissue from patients with chronic periodontitis (CP), patients with CP and type 2 diabetes mellitus (T2DM), and healthy individuals. Methods: The study includes 50 individuals: 10 h...

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Bibliographic Details
Published in:Journal of periodontology (1970) 2015-07, Vol.86 (7), p.882-889
Main Authors: Ghallab, Noha A., Amr, Eman M., Shaker, Olfat G.
Format: Article
Language:English
Subjects:
Adipokines
Adult
Biopsy - methods
Blood Glucose - analysis
Chronic Periodontitis - complications
Chronic Periodontitis - metabolism
Cytokines - analysis
Dental Plaque Index
Dentistry
diabetes mellitus
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - complications
Enzyme-Linked Immunosorbent Assay
Female
Gingiva - chemistry
Glycated Hemoglobin A - analysis
Humans
Leptin - analysis
Male
Middle Aged
molecular biology
Nicotinamide Phosphoribosyltransferase - analysis
Periodontal Attachment Loss - complications
Periodontal Attachment Loss - metabolism
Periodontal Index
Periodontal Pocket - complications
Periodontal Pocket - metabolism
periodontitis
polymerase chain reaction
Real-Time Polymerase Chain Reaction
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Summary:Background: The aim of this study is to investigate the protein and gene expression of leptin and visfatin in gingival tissue from patients with chronic periodontitis (CP), patients with CP and type 2 diabetes mellitus (T2DM), and healthy individuals. Methods: The study includes 50 individuals: 10 healthy individuals, 20 patients with CP, and 20 patients with CP and T2DM. Plaque index, gingival index, probing depth, and clinical attachment loss were measured, and gingival biopsies were obtained. Leptin and visfatin protein expression in gingival tissues was determined using enzyme‐linked immunosorbent assay, and messenger RNA (mRNA) expression was measured via real‐time polymerase chain reaction. Results: The highest leptin mRNA and protein expression was observed in the control group and was significantly (P ≤0.05) different from the CP and CP+T2DM groups. Gingival tissues from patients with CP and T2DM had a significant increase in visfatin and a decrease in leptin gene and protein expression (P
ISSN:0022-3492
1943-3670
DOI:10.1902/jop.2015.140434