Impact of Oral Commensal Bacteria on Degradation of Periodontal Connective Tissue in Mice

Background: Innate and adaptive immunosurveillance mechanisms in response to the normal commensal bacteria can affect periodontal innate defense status. However, it is still unclear how commensal bacteria contribute to the inflammatory responses of junctional epithelium (JE) and periodontal connecti...

Full description

Saved in:
Bibliographic Details
Published in:Journal of periodontology (1970) 2015-07, Vol.86 (7), p.899-905
Main Authors: Irie, Koichiro, Tomofuji, Takaaki, Ekuni, Daisuke, Morita, Manabu, Shimazaki, Yoshihiro, Darveau, Richard P.
Format: Article
Language:English
Subjects:
Animals
Azo Compounds
Bacteria - immunology
Cell Movement - immunology
Collagen - analysis
Collagen - ultrastructure
Coloring Agents
Connective tissue
Connective Tissue - immunology
Connective Tissue - microbiology
Dentistry
Epithelial Attachment - immunology
Epithelial Attachment - microbiology
Germ-Free Life
immunity, innate
Immunity, Innate - immunology
inflammation
Intercellular Adhesion Molecule-1 - analysis
Matrix Metalloproteinase 13 - analysis
Matrix Metalloproteinase 8 - analysis
Mice
Microscopy, Polarization - methods
Mouth - microbiology
Neutrophils - immunology
periodontitis
Periodontium - immunology
Periodontium - microbiology
Receptor, Fibroblast Growth Factor, Type 1 - analysis
Specific Pathogen-Free Organisms
Symbiosis - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: Innate and adaptive immunosurveillance mechanisms in response to the normal commensal bacteria can affect periodontal innate defense status. However, it is still unclear how commensal bacteria contribute to the inflammatory responses of junctional epithelium (JE) and periodontal connective tissue (PCT). The aim of the present study is to investigate the contribution of commensal bacteria on inflammatory responses in JE and PCT in mice. Methods: The periodontal tissue of germ‐free (GF) and specific‐pathogen‐free (SPF) mice were compared at age 11 to 12 weeks (n = 6 per group). In this study, the number of neutrophils and expression of intercellular adhesion molecule (ICAM)‐1, fibroblast growth factor receptor (FGFR)‐1, matrix metalloproteinase (MMP)‐1, and MMP‐8 within the JE and the PCT are evaluated. The collagen density was also determined in PCT stained with picrosirius red (PSR). PSR staining combined with or without polarized light microscopy has been used to assess the organization and maturation of collagen matrix. Results: In the present findings, the area of JE in SPF mice was significantly greater than that in GF mice (P
ISSN:0022-3492
1943-3670
DOI:10.1902/jop.2015.150006