Alterations in Human Liver Metabolome during Prolonged Cryostorage

Tissue metabolomics requires high sample quality that crucially depends on the biobanking storage protocol. Hence, we systematically analyzed the influence of realistic storage scenarios on the liver metabolome with different storage temperatures and repeated transfer of samples between storage and...

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Bibliographic Details
Published in:Journal of proteome research 2015-07, Vol.14 (7), p.2758-2768
Main Authors: Abuja, Peter M, Ehrhart, Friederike, Schoen, Uwe, Schmidt, Tomm, Stracke, Frank, Dallmann, Guido, Friedrich, Torben, Zimmermann, Heiko, Zatloukal, Kurt
Format: Article
Language:English
Subjects:
Chromatography, Liquid
Cryopreservation
Humans
Liver - metabolism
Mass Spectrometry
Metabolomics
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Summary:Tissue metabolomics requires high sample quality that crucially depends on the biobanking storage protocol. Hence, we systematically analyzed the influence of realistic storage scenarios on the liver metabolome with different storage temperatures and repeated transfer of samples between storage and retrieval environments, simulating the repeated temperature changes affecting unrelated samples stored in the same container as the sample that is to be retrieved. By cycling between storage (−80 °C freezer, liquid nitrogen, cold nitrogen gas) and retrieval (room temperature, −80 °C), assuming three cycles per day and sample, we simulated biobank storage between 3 months and 10 years. Liver tissue metabolome was analyzed by liquid chromatography/mass spectrometry. Most metabolite concentrations changed
ISSN:1535-3893
1535-3907
DOI:10.1021/acs.jproteome.5b00025