P(CH sub(2)OH) sub(3)-polyetheramine - Derived polymeric films for enzyme immobilization

Polymer films formed by the condensation of polyetheramines with P(CH sub(2)OH) sub(3) were synthesized and covalently coupled to the enzyme urease via the intrinsic reactive P-CH sub(2)OH hydroxyl groups. Urease activities of 1,140 U g super(-1) and 5.5 U cm super(-2) of polymer film were achieved...

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Bibliographic Details
Published in:Enzyme and microbial technology 1995-01, Vol.17 (8), p.746-750
Main Authors: Bonnington, L S, Henderson, W, Petach, H H
Format: Article
Language:English
Online Access:Get full text
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Summary:Polymer films formed by the condensation of polyetheramines with P(CH sub(2)OH) sub(3) were synthesized and covalently coupled to the enzyme urease via the intrinsic reactive P-CH sub(2)OH hydroxyl groups. Urease activities of 1,140 U g super(-1) and 5.5 U cm super(-2) of polymer film were achieved without any additional coupling agents. Upon oxidation of the phosphorus, the intrinsic hydroxyl groups were inactivated and the resulting urease activities decreased by over 98%.
ISSN:0141-0229