Fluorometric imaging methods for palladium and platinum and the use of palladium for imaging biomolecules

Neither palladium nor platinum is an endogenous biological metal. Imaging palladium in biological samples, however, is becoming increasingly important because bioorthogonal organometallic chemistry involves palladium catalysis. In addition to being an imaging target, palladium has been used to fluor...

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Bibliographic Details
Published in:Chemical Society reviews 2015-07, Vol.44 (14), p.4769-4791
Main Authors: Tracey, Matthew P, Pham, Dianne, Koide, Kazunori
Format: Article
Language:English
Subjects:
Animals
Escherichia coli
HeLa Cells
Humans
Microscopy, Fluorescence - methods
Palladium - analysis
Palladium - chemistry
Platinum - analysis
Platinum - chemistry
Zebrafish
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Summary:Neither palladium nor platinum is an endogenous biological metal. Imaging palladium in biological samples, however, is becoming increasingly important because bioorthogonal organometallic chemistry involves palladium catalysis. In addition to being an imaging target, palladium has been used to fluorometrically image biomolecules. In these cases, palladium species are used as imaging-enabling reagents. This review article discusses these fluorometric methods. Platinum-based drugs are widely used as anticancer drugs, yet their mechanism of action remains largely unknown. We discuss fluorometric methods for imaging or quantifying platinum in cells or biofluids. These methods include the use of chemosensors to directly detect platinum, fluorescently tagging platinum-based drugs, and utilizing post-labeling to elucidate distribution and mode of action. Palladium and platinum metals have been used to facilitate novel bioimaging methods.
ISSN:0306-0012
1460-4744
DOI:10.1039/c4cs00323c