Versatility of Microbial Transglutaminase

Although microbial transglutaminases (mTGs) were initially discovered to offset the cost of producing mammalian transglutaminases for food applications, they have quickly become important tools in research and biotechnology. Today, mTGs are utilized for a large number of applications to conjugate pr...

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Bibliographic Details
Published in:Bioconjugate chemistry 2014-05, Vol.25 (5), p.855-862
Main Author: Strop, Pavel
Format: Article
Language:English
Subjects:
Antibodies - chemistry
Antibodies - metabolism
Antineoplastic Agents - chemistry
Antineoplastic Agents - metabolism
Antineoplastic Agents - therapeutic use
Biotechnology
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - metabolism
Molecules
Neoplasms - drug therapy
Neoplasms - metabolism
Peptides
Polymers
Polymers - chemistry
Polymers - metabolism
Proteins
Streptomyces - enzymology
Substrate Specificity
Transglutaminases - chemistry
Transglutaminases - metabolism
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Summary:Although microbial transglutaminases (mTGs) were initially discovered to offset the cost of producing mammalian transglutaminases for food applications, they have quickly become important tools in research and biotechnology. Today, mTGs are utilized for a large number of applications to conjugate proteins and peptides to small molecules, polymers, surfaces, and DNA, as well as to other proteins. It is important to know how to maximize the advantages of the enzymatic approach and avoid undesired cross-linking. This review focuses on the versatility of transglutaminases in the field of bioconjugation and covers recent developments in utilizing mTG for generating antibody drug conjugates (ADCs) for therapeutic applications.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc500099v