Novel properties of antimicrobial peptide anoplin

•Amidated and non-amidated forms of anoplin have strong antifungal activity.•Treatment with both forms of anoplin protects B. napus from L. maculans infection.•Both forms of anoplin activate defence gene expression in cotyledons of B. napus. Antimicrobial decapeptide anoplin was tested for its antif...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2014-02, Vol.444 (4), p.520-524
Main Authors: Jindrichova, Barbora, Burketova, Lenka, Novotna, Zuzana
Format: Article
Language:English
Subjects:
Amides - chemistry
Amides - pharmacology
Anoplin
Antifungal
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Antimicrobial Cationic Peptides - chemistry
Antimicrobial Cationic Peptides - pharmacology
Antimicrobial peptide
Ascomycota - drug effects
Brassica napus
Brassica napus - genetics
Brassica napus - microbiology
Gene Expression Regulation, Plant - drug effects
Leptosphaeria maculans
Plant Diseases - microbiology
Plant Diseases - prevention & control
Wasp Venoms - chemistry
Wasp Venoms - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Amidated and non-amidated forms of anoplin have strong antifungal activity.•Treatment with both forms of anoplin protects B. napus from L. maculans infection.•Both forms of anoplin activate defence gene expression in cotyledons of B. napus. Antimicrobial decapeptide anoplin was tested for its antifungal activity against plant pathogen Leptosphaeria maculans and protection of Brassica napus plants from disease. To reveal the mode of action of the peptide, a natural form of anoplin amidated on C-terminus (ANP-NH2), and its carboxylated analog (ANP-OH) were used in the study. We demonstrated strong antifungal activity of anoplin in vitro regardless C-terminus modification. In addition we show that both ANP-NH2 and ANP-OH induce expression of defence genes in B. napus and protects plants from L. maculans infection. The results indicate that the amidation of anoplin is not essential for its antifungal and plant defence stimulating activities.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2014.01.097