Isolation of pathogenic microorganisms from burn patients admitted in Dhaka Medical College and Hospital and demonstration of their drug-resistance traits

• Published in: Asian Pacific journal of tropical disease 2014-10, Vol.4 (5), p.402-407
• Main Authors: Alam, Syed Md Shariar, Kalam, Md. Abul, Munna, Md. Sakil, Munshi, Saurab Kishore, Noor, Rashed
• Format: Article
• Language: English
• Subjects: Antimicrobial activity; Burn wounds; Enterobacter; Escherichia coli; Microorganisms; Pseudomonas; Public health; Staphylococcus aureus
• ISSN: 2222-1808; 2222-1808
• DOI: 10.1016/S2222-1808(14)60596-X

To isolate and quantify the microflora from the burn patients admitted in the Division of Plastic Surgery and Burns outdoor patients in Dhaka Medical College Hospital, Bangladesh. Thirty wound surface swab samples of first and second degree burn patients were collected and the microbial analysis as well the study of antibacterial susceptibility was conducted. Microbial inhibitory concentration of tobramycin was tested to be applied as effective antimicrobial agent in burn patients. Activity of four disinfectants was also tested against the pathogens. Among all samples, 28 was found to be populated with the total viable bacteria up to 107 CFU/mL. The predominant pathogen was Pseudomonas spp. followed by Staphylococcus aureus and Kebsiella spp. Three of the samples harbored Enterobacter spp. while 2 were found to be proliferated with Escherichia coli. Most of the pathogens were found to be drug-resistant while several isolates were noted to be multi-drug resistant. Dettol partly showed efficacy among the tested disinfectants to prevent pathogenic proliferation. Huge bacterial onset with an alarming threat of multidrug resistance would potentially raise the necessity of proper care and management of burn wound patients in hospital.