Cord formation in BACTEC(TM) medium aids rapid identification of Mycobacterium tuberculosis complex

Mycobacterium tuberculosis complex (MTC) organisms form serpentine cords in fluid culture medium. Reporting of a presumptive identification of MTC based on cording allows rapid identification of patients with tuberculosis. A total of 612 positive mycobacterial cultures from 316 patients over 3 years...

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Bibliographic Details
Published in:The international journal of tuberculosis and lung disease 2015-06, Vol.19 (6), p.709-710
Main Authors: O'Hara, G A, Abdullah, S, Creer, D D, Elsaghier, A F
Format: Article
Language:English
Subjects:
Bacteriological Techniques
Culture Media
Feasibility Studies
Humans
Mycobacterium tuberculosis
Mycobacterium tuberculosis - growth & development
Mycobacterium tuberculosis - isolation & purification
Predictive Value of Tests
Reproducibility of Results
Retrospective Studies
Time Factors
Tuberculosis - diagnosis
Tuberculosis - microbiology
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Summary:Mycobacterium tuberculosis complex (MTC) organisms form serpentine cords in fluid culture medium. Reporting of a presumptive identification of MTC based on cording allows rapid identification of patients with tuberculosis. A total of 612 positive mycobacterial cultures from 316 patients over 3 years (2008-2010) were evaluated for the presence of cord formation. Cording was identified in 426 (69.6%) specimens, while the reference laboratory confirmed M. tuberculosis in 424 specimens (69.3%). Sensitivity of the test in our laboratory was 99.1% (95%CI 97.4-99.7) and specificity was 96.8% (95%CI 92.8-98.7). Presumptive identification of M. tuberculosis by the presence of cording formation is both sensitive and specific.
ISSN:1027-3719
1815-7920
DOI:10.5588/ijtld.14.0586