Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures

We evaluated the feasibility of same-day routine aerobic bacterial identification using the following procedures: Picking colonies from 4 and 6 h incubated subculture from positive blood culture bottle and analyzing them by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry...

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Bibliographic Details
Published in:Indian journal of medical microbiology 2014-10, Vol.32 (4), p.419-422
Main Authors: Hong, Sung Kuk, Chang, Beung Ki, Song, Sang Hoon, Kim, Eui-Chong
Format: Article
Language:English
Subjects:
Bacteria
Blood
Blood culture
Desorption
Gram-negative bacteria
Identification systems
Ions
Laboratories
Lasers
MALDI-TOF MS
Mass spectrometry
Methods
rapid bacterial identification
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Summary:We evaluated the feasibility of same-day routine aerobic bacterial identification using the following procedures: Picking colonies from 4 and 6 h incubated subculture from positive blood culture bottle and analyzing them by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The matched identification rate of this procedure at the species level was 80.6% (141/175) for the 4-h cultures compared with overnight cultures and 90.9% (159/175) for the 6-h cultures. Thus, our technique provides an easy and rapid method for identification of aerobic bacteria in routine clinical microbiology laboratories.
ISSN:0255-0857
1998-3646
DOI:10.4103/0255-0857.142261