Transgenic tomato overexpressing ath-miR399d improves growth under abiotic stress conditions

Phosphorus (P) is an essential element required for plant growth and development. Abiotic stresses, such as cold and P deficiency cause adverse effects on growth of plants, inhibit accumulation or translocation of mineral nutrients such as P, and often constrain the productivity and quality of crops...

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Bibliographic Details
Published in:Russian journal of plant physiology 2015-05, Vol.62 (3), p.360-366
Main Authors: Gao, N, Qiang, X. M, Zhai, B. N, Min, J, Shi, W. M
Format: Article
Language:English
Subjects:
adverse effects
Biomedical and Life Sciences
cold
cold stress
crops
drought
gene overexpression
genes
genetically modified organisms
growth retardation
homeostasis
Life Sciences
Lycopersicon esculentum
nutrients
phosphorus
plant growth
Plant Physiology
Plant Sciences
Research Papers
salinity
salt stress
soil
Solanum
Solanum lycopersicum
temperature
tomatoes
toxicity
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Summary:Phosphorus (P) is an essential element required for plant growth and development. Abiotic stresses, such as cold and P deficiency cause adverse effects on growth of plants, inhibit accumulation or translocation of mineral nutrients such as P, and often constrain the productivity and quality of crops. miR399 as a long-distance signal controls systemic phosphate homeostasis by modulating phosphate uptake and transport. However, overexpression of miR399 causes P toxicity and results in retarded growth. Thus, we constructed a transgenic tomato (Solanum lycopersicum L., C1), which contains foreign gene ath-miR399d under the control of rd29A promoter. In the transgenic C1 expression of the miR399d gene showed a stress-inducible pattern. Results showed that miR399d was moderately up-regulated in response to salinity and cold stresses. In soil, dry weight was enhanced in transgenic tomato under low temperature and P deficiency conditions. The transgenic tomato has potential to improve growth when it is exposed to other abiotic stresses such as salinity and drought.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443715030061