Effect of transient blood glucose increases after oral glucose intake on the human urinary proteome

Purpose Unlike blood, which has mechanisms to maintain homeostasis, urine is more likely to reflect changes in the body. However, the urinary proteome is affected by many factors such as diet, medication, daily activities. Transient increase of blood glucose frequently occurs after the consumption o...

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Bibliographic Details
Published in:Proteomics. Clinical applications 2015-06, Vol.9 (5-6), p.618-622
Main Authors: Li, Menglin, Zhao, Mindi, Gao, Youhe
Format: Article
Language:English
Subjects:
Adult
Blood Glucose
Female
Glucose
Glucose Tolerance Test
High blood glucose
Humans
Hypoglycemia
Influence factor
Male
Peptide Fragments - urine
Proteome - metabolism
Tandem Mass Spectrometry
Urinary proteome
Urine
Young Adult
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Summary:Purpose Unlike blood, which has mechanisms to maintain homeostasis, urine is more likely to reflect changes in the body. However, the urinary proteome is affected by many factors such as diet, medication, daily activities. Transient increase of blood glucose frequently occurs after the consumption of food and may be an interfering factor in biomarker studies. Experimental design In this study, a transient increase of blood glucose level was achieved by performing an oral glucose tolerance test on nine healthy human volunteers. Urine samples before and after the test were collected and analyzed by LC‐MS/MS. Results According to quantification using Progenesis LC‐MS software, only the protein neutrophil defensin 1, which was reported to participate in reducing blood glucose levels, was increased in the urine after glucose intake; and almost all urinary proteins may not be affected. Conclusions and clinical relevance Transient increase of blood glucose levels may not be a major interfering factor in biomarker studies, at least with current levels of identification and quantification.
ISSN:1862-8346
1862-8354
DOI:10.1002/prca.201400174