A tadpole-shaped gene carrier with distinct phase segregation in a ternary polymeric micelle

A distinct tadpole-shaped nanostructure characterized by a spherical head and an extended shaft was identified in a single plasmid DNA (pDNA)-based polymeric micelle. The tadpole-shaped structure was constructed by adding anionic chondroitin sulfate (CS) to the rod-shaped polyplex micelle containing...

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Bibliographic Details
Published in:Soft matter 2015-04, Vol.11 (14), p.2718-2722
Main Authors: Chen, Qixian, Osada, Kensuke, Pennisi, Matthew, Uchida, Satoshi, Tockary, Theofilus A, Dirisala, Anjaneyulu, Li, Yanmin, Takeda, Kaori M, Oniyanagi, Satoshi, Itaka, Keiji, Kataoka, Kazunori
Format: Article
Language:English
Subjects:
Block copolymers
Chondroitin Sulfates - chemistry
Deoxyribonucleic acid
DNA, Single-Stranded - chemistry
DNA, Single-Stranded - metabolism
Electrostatics
Gene Transfer Techniques
Genes
Micelles
Nanostructure
Nanostructures - chemistry
Plasmids - genetics
Plasmids - metabolism
Polyamines - chemistry
Polyethylene Glycols - chemistry
Polymers - chemistry
Segregations
Self assembly
Spectrometry, X-Ray Emission
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Summary:A distinct tadpole-shaped nanostructure characterized by a spherical head and an extended shaft was identified in a single plasmid DNA (pDNA)-based polymeric micelle. The tadpole-shaped structure was constructed by adding anionic chondroitin sulfate (CS) to the rod-shaped polyplex micelle containing a single pDNA molecule packaged by the PEG-polycation block copolymer through their electrostatic self-assembly. The complex consequently developed a novel structure composed of segregated domains of the CS-rich inflated head and CS-poor folded DNA tail. Hence, this tadpole structure can be regarded as evidence that distinct phase segregation occurred in a single polymeric micelle containing pDNA.
ISSN:1744-683X
1744-6848
DOI:10.1039/c4sm02850c