HINFI POLYMORPHISM OF PIT-1 GENE IN SLOVAK SPOTTED CATTLE

In this study has been Pit-1 gene detected as the pituitary specific transcription factor that regulates the expression of the growth hormone and prolactin genes in the anterior pituitary. A total of 110 Slovak Spotted cows were genotyped for polymorphism of Pit-1/HinfI gene in exon 6 on bovine chro...

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Bibliographic Details
Published in:Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2013-01 (Special Issue 1 - Part B), p.1883-1890
Main Authors: Moravcíková, Nina, Trakovická, Anna, Miluchová, Martina, Bujko, Jozef, Navrátilová, Alica
Format: Article
Language:English
Subjects:
Cattle
Enzymes
Genes
Genotype & phenotype
Growth hormones
Mutation
Pituitary gland
Polymorphism
Transcription factors
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Summary:In this study has been Pit-1 gene detected as the pituitary specific transcription factor that regulates the expression of the growth hormone and prolactin genes in the anterior pituitary. A total of 110 Slovak Spotted cows were genotyped for polymorphism of Pit-1/HinfI gene in exon 6 on bovine chromosome 1 by using polymerase chain reaction and restriction fragment length polymorphism methods. Digestion of PCR products with restriction enzyme HinfI revealed two alleles: allele A gave one fragments, 260 bp in length and allele B gave two fragments of 190 and 70 bp. The predominant allele was B with observed frequency 0.7045. In population were detected all three genotypes AA, AB and BB. The most frequent was heterozygous genotype AB with observed frequency 0.417. The population was in Hardy-Weinberg equilibrium.
ISSN:1338-5178