Isolation and characterization of restriction endonuclease Ec/HKI from an Enterobacter cloacae strain

An Enterobacter cloacae strain, producing restriction enzyme Ec/HKI, was isolated from a decaying potato. The enzyme is an isoschizomer of Eam11051, which recognizes and cleaves GAACNN!NNGTC. Ec/HKI was produced at high activity (4 x 10 super(4) U/g wet cells) and was purified from contaminants whic...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1994, Vol.10 (1), p.30-32
Main Authors: Chan, Ho-Yin, Chan, Ying-Chi, Kam, Kai-Man, Shaw, Pang-Chui
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enterobacter cloacae
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Improved methods for extraction and purification of enzymes
Methods. Procedures. Technologies
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Summary:An Enterobacter cloacae strain, producing restriction enzyme Ec/HKI, was isolated from a decaying potato. The enzyme is an isoschizomer of Eam11051, which recognizes and cleaves GAACNN!NNGTC. Ec/HKI was produced at high activity (4 x 10 super(4) U/g wet cells) and was purified from contaminants which interfere with restriction digestion by passing the cell lysate through DEAE-Sephacel and heparin columns. Activity was optimal at 37 degree C in a medium salt buffer.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF00357558