Platelet microparticles are internalized in neutrophils via the concerted activity of 12-lipoxygenase and secreted phospholipase A₂-IIA

• Published in: Proceedings of the National Academy of Sciences - PNAS 2015-07, Vol.112 (27), p.E3564-E3573
• Main Authors: Duchez, Anne-Claire, Boudreau, Luc H., Naika, Gajendra S., Bollinger, James, Belleannée, Clémence, Cloutier, Nathalie, Laffont, Benoit, Mendoza-Villarroel, Raifish E., Lévesque, Tania, Rollet-Labelle, Emmanuelle, Rousseau, Matthieu, Allaeys, Isabelle, Tremblay, Jacques J., Poubelle, Patrice E., Lambeau, Gérard, Pouliot, Marc, Provost, Patrick, Soulet, Denis, Gelb, Michael H., Boilard, Eric
• Format: Article
• Language: English
• Subjects: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - metabolism; Animals; Arachidonate 12-Lipoxygenase - genetics; Arachidonate 12-Lipoxygenase - metabolism; Arthritis, Experimental - genetics; Arthritis, Experimental - metabolism; Arthritis, Rheumatoid - genetics; Arthritis, Rheumatoid - metabolism; Biological Sciences; Blood Platelets - enzymology; Blood Platelets - metabolism; Cell Line; Cell-Derived Microparticles - enzymology; Cell-Derived Microparticles - metabolism; Cell-Derived Microparticles - ultrastructure; Cells, Cultured; Endocytosis; Group II Phospholipases A2 - genetics; Group II Phospholipases A2 - metabolism; Humans; Immunoblotting; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Microscopy, Confocal; Microscopy, Electron; Mitochondria - metabolism; Mitochondria - ultrastructure; Neutrophils - metabolism; Neutrophils - ultrastructure; PNAS Plus; Reverse Transcriptase Polymerase Chain Reaction; RNA - genetics; RNA - metabolism; Synovial Fluid - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1507905112

Platelets are anucleated blood elements highly potent at generating extracellular vesicles (EVs) called microparticles (MPs). Whereas EVs are accepted as an important means of intercellular communication, the mechanisms underlying platelet MP internalization in recipient cells are poorly understood. Our lipidomic analyses identified 12(S)-hydroxyeicosatetranoic acid [12(S)-HETE] as the predominant eicosanoid generated by MPs. Mechanistically, 12(S)-HETE is produced through the concerted activity of secreted phospholipase A₂ IIA (sPLA₂-IIA), present in inflammatory fluids, and platelet-type 12-lipoxygenase (12-LO), expressed by platelet MPs. Platelet MPs convey an elaborate set of transcription factors and nucleic acids, and contain mitochondria. We observed that MPs and their cargo are internalized by activated neutrophils in the endomembrane system via 12(S)-HETE. Platelet MPs are found inside neutrophils isolated from the joints of arthritic patients, and are found in neutrophils only in the presence of sPLA₂-IIA and 12-LO in an in vivo model of autoimmune inflammatory arthritis. Using a combination of genetically modified mice, we show that the coordinated action of sPLA₂-IIA and 12-LO promotes inflammatory arthritis. These findings identify 12(S)-HETE as a trigger of platelet MP internalization by neutrophils, a mechanism highly relevant to inflammatory processes. Because sPLA₂-IIA is induced during inflammation, and 12-LO expression is restricted mainly to platelets, these observations demonstrate that platelet MPs promote their internalization in recipient cells through highly regulated mechanisms.