The skin migratory stage of the schistosomulum of Schistosoma mansoni has a surface showing greater permeability and activity in membrane internalisation than other forms of skin or mechanical schistosomula

Skin schistosomula can be prepared by collecting them after isolated mouse skin have been penetrated by cercariae in vitro. The schistosomula can also migrate out of isolated mouse skin penetrated by cercariae in vitro and from mouse skin penetrated by cercariae in vivo. Schistosomula can also be pr...

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Bibliographic Details
Published in:Parasitology 2015-08, Vol.142 (9), p.1143-1151
Main Authors: DE JESUS JEREMIAS, WANDER, DA CUNHA MELO, JOSE RENAN, BABA, ELIO HIDEO, COELHO, PAULO MARCOS ZECH, KUSEL, JOHN ROBERT
Format: Article
Language:English
Subjects:
Animals
Fluorescent Dyes
Isoquinolines - chemistry
Mice
Movement
Permeability
Schistosoma mansoni - physiology
Schistosomiasis mansoni - parasitology
Schistosomiasis mansoni - pathology
Skin
Skin - parasitology
Skin - pathology
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Summary:Skin schistosomula can be prepared by collecting them after isolated mouse skin have been penetrated by cercariae in vitro. The schistosomula can also migrate out of isolated mouse skin penetrated by cercariae in vitro and from mouse skin penetrated by cercariae in vivo. Schistosomula can also be produced from cercariae applied through a syringe or in a vortex. When certain surface properties of the different forms of schistosomula were compared, those migrating from mouse skin penetrated by cercariae in vivo or in vitro had greatly increased permeability to membrane impermeant molecules such as Lucifer yellow and high molecular weight dextrans. These migrating forms also possessed surfaces which showed greatly enhanced uptake into internal membrane vesicles of the dye FM 143, a marker for endocytosis. This greatly enhanced activity and permeability of the surfaces of tissue migrating schistosomula is likely to be of great importance in the adaptation to the new host.
ISSN:0031-1820
1469-8161
1469-8161
DOI:10.1017/S0031182015000335