Production of catechols and muconic acids from various aromatics by the styrene-degrader Rhodococcus rhodochrous NCIMB 13259

Intact cells of Rhodococcus rhodochrous NCIMB 13259 which had been grown in the presence of styrene were used to produce metabolic intermediates from other aromatic substrates in the presence of 3-fluorocatechol as an inhibitor of catechol oxygenase. Toluene and ethylbenzene gave 3-methylcatechol an...

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Bibliographic Details
Published in:Biotechnology letters 1994-05, Vol.16 (5), p.513-516
Main Authors: WARHURST, A. M, CLARKE, K. F, HILL, R. A, HOLT, R. A, FEWSON, C. A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Mission oriented research
Physiology and metabolism
Rhodococcus rhodochrous
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Summary:Intact cells of Rhodococcus rhodochrous NCIMB 13259 which had been grown in the presence of styrene were used to produce metabolic intermediates from other aromatic substrates in the presence of 3-fluorocatechol as an inhibitor of catechol oxygenase. Toluene and ethylbenzene gave 3-methylcatechol and 3-ethylcatechol respectively. The enol form of 3-acetylcatechol was produced from acetophenone and 1-phenylethanol. Benzoic acid was produced from cinnamic acid. This organism cannot metabolise muconic acids, and methylmuconic acid and ethylmuconic acid accumulated under appropriate conditions. 2-Fluoromuconic acid was also produced from the added 3-fluorocatechol. This organism provides a convenient tool for producing catechols and muconic acids from a variety of substrates without need for genetic manipulation.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01023335