Molecular forms of lipases and their localization in the fungus Rhizopus microsporus by immuno-electron microscopy

Several lipases differing in their molecular masses (24, 32, 43, 66 and 98 kDa and 28, 40, 45 and 69 kDa) were found in Rhizopus microsporus UzLT-4B and UzLT-5C, respectively. The lipases in each strain were immunologically related. Strain UzLT-5C grown on a medium with lipid substrate secreted lipa...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1994-07, Vol.10 (4), p.367-373
Main Authors: Diyorov, J K, Lusta, K A, Tsiomenko, A B, Kulaev, I S
Format: Article
Language:English
Subjects:
Rhizopus microsporus
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Summary:Several lipases differing in their molecular masses (24, 32, 43, 66 and 98 kDa and 28, 40, 45 and 69 kDa) were found in Rhizopus microsporus UzLT-4B and UzLT-5C, respectively. The lipases in each strain were immunologically related. Strain UzLT-5C grown on a medium with lipid substrate secreted lipases of 32, 66 and 98 kDa whereas strain UzLT-4B produced lipases of 45 and 69 kDa on the same medium. Immuno-electron microscopy indicated that the intracellular lipases were in peripheral zones of the hyphae, primarily in the periplasm and adjacent vesicles. Immobilization in Ca-alginate gel revealed unusual structures in the cell wall. These structures accumulated lipases and, apparently, exported the enzymes out of the cell.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF00144453