Modification of carbonic anhydrase activity by antisense and over-expression constructs in transgenic tobacco

The activity and location of carbonic anhydrase has been modified by transformation of tobacco with antisense and over-expression constructs. Antisense expression resulted in the inhibition of up to 99% of carbonic anhydrase activity but had no significant impact on net CO2 assimilation. Stomatal co...

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Bibliographic Details
Published in:Plant molecular biology 1994-06, Vol.25 (3), p.377-385
Main Authors: Majeau, N. (Toronto Univ., Toronto, Ont. (Canada). Dept. of Botany), Arnoldo, M.A, Coleman, J.R
Format: Article
Language:English
Subjects:
Antisense Elements (Genetics)
Carbon Dioxide - metabolism
CARBONATE DEHYDRATASE
CARBONATE DESHYDRATASE
CARBONATO DESHIDRATASA
Carbonic Anhydrases - genetics
Carbonic Anhydrases - metabolism
CHLOROPLASTE
CHLOROPLASTS
CLOROPLASTO
FOTOSINTESIS
NICOTIANA
Nicotiana - enzymology
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana tabacum
PHOTOSYNTHESE
PHOTOSYNTHESIS
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified
Plants, Toxic
Transformation, Genetic
TRANSGENIC PLANTS
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Summary:The activity and location of carbonic anhydrase has been modified by transformation of tobacco with antisense and over-expression constructs. Antisense expression resulted in the inhibition of up to 99% of carbonic anhydrase activity but had no significant impact on net CO2 assimilation. Stomatal conductance and susceptibility to water stress appeared to increase in response to the decline in carbonic anhydrase activity. An over-expression construct designed to increase cytosolic carbonic anhydrase abundance resulted in a significant increase in net activity, a small increase in stomatal conductance but little impact on CO2 assimilation. Chloroplastic carbonic anhydrase activity was enhanced by the expression of an additional construct which targeted the polypeptide to the organelle. The increase in chloroplastic carbonic anhydrase appeared to be accompanied by a concomitant increase in Rubisco activity.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00043867