Cytoplasmic DNAs and nuclear rDNA restriction fragment length polymorphisms in commercial witloof chicories

Restriction fragment length polymorphisms of cytoplasmic DNAs and nuclear rDNA were analyzed in several Cichorium intybus genotypes, comprising four white inbred lines, eight red witloof experimental lines, and a number of F1 hybrids derived from two white parents. Chloroplast and mitochondrial rest...

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Bibliographic Details
Published in:Theoretical and applied genetics 1995-08, Vol.91 (3), p.505-509
Main Authors: Bellamy, A, Mathieu, C, Vedel, F, Bannerot, H. (Paris-Sud Univ., Orsay (France). Centre de Recherche sur les Plantes. URA Centre National da la Recherche Scientifique)
Format: Article
Language:English
Subjects:
ADN
Biological and medical sciences
CHLOROPLASTE
CICHORIUM INTYBUS
Classical genetics, quantitative genetics, hybrids
CLOROPLASTO
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
Fundamental and applied biological sciences. Psychology
GENETICA MITOCONDRIAL
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE MITOCHONDRIALE
GENOTIPOS
GENOTYPE
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Pteridophyta, spermatophyta
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Vegetals
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Summary:Restriction fragment length polymorphisms of cytoplasmic DNAs and nuclear rDNA were analyzed in several Cichorium intybus genotypes, comprising four white inbred lines, eight red witloof experimental lines, and a number of F1 hybrids derived from two white parents. Chloroplast and mitochondrial restriction patterns led to the distinction between two different cytoplasms, called 1 and 2. Southern hybridization using a nuclear rDNA probe revealed that all the lines possessed two types of rDNA repeat units. The shortest unit was 10 kb and was common to all lines. The largest rDNA repeat unit was 10.5 kb in lines 1 and 10.4 kb in lines 2. In addition, a sequence heterogeneity between the 10.5- and 10.4-kb rDNA repeat units was revealed by Sac1 digestion. A 10-kb rDNA unit was successively cloned, mapped, and used as a probe to check the genetic purity of F1 hybrid seeds between line 1 and 2 white parents. A 30% average percentage of impurities, originating both from selfing and full-sib crossing, in different open-pollinated hybrid samples were found.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00222980