Endothelin stimulates prostaglandin endoperoxide synthase-2 mRNA expression and protein synthesis through a tyrosine kinase-signaling pathway in rat mesangial cells

We evaluated the role of endothelin-1 (ET-1), a vasoconstrictor peptide, to regulate prostaglandin endoperoxide synthase (PGHS)-1 and -2 gene expression and protein synthesis in cultured rat mesangial cells (MC). ET-1 induced mRNA for PGHS-2 but not PGHS-1 and also stimulated protein accumulation of...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1994-09, Vol.269 (36), p.22574-22580
Main Authors: Kester, M, Coroneos, E, Thomas, P J, Dunn, M J
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Cycloheximide - pharmacology
Dactinomycin - pharmacology
Dexamethasone - pharmacology
Endothelins - pharmacology
Gene Expression - drug effects
Glomerular Mesangium - drug effects
Glomerular Mesangium - enzymology
Heparin - pharmacology
Isoenzymes - biosynthesis
Kinetics
Prostaglandin-Endoperoxide Synthases - biosynthesis
Protein Biosynthesis - drug effects
Rats
RNA, Messenger - biosynthesis
Time Factors
Transcription, Genetic - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We evaluated the role of endothelin-1 (ET-1), a vasoconstrictor peptide, to regulate prostaglandin endoperoxide synthase (PGHS)-1 and -2 gene expression and protein synthesis in cultured rat mesangial cells (MC). ET-1 induced mRNA for PGHS-2 but not PGHS-1 and also stimulated protein accumulation of PGHS-2 but not PGHS-1 in MC. ET-1 induction of PGHS-2 protein was accompanied by a sustained enhancement of enzymatic activity assessed by conversion of arachidonic acid to prostaglandin E2. The ET-1-stimulated PGHS-2 expression was reduced with protein tyrosine kinase but not with protein kinase C inhibitors or in protein kinase C-depleted cells. Both dexamethasone and heparin reduced ET-1-activated PGHS-2 mRNA expression and protein formation. We conclude that in MC, ET-1 induces PGHS-2 through a protein tyrosine kinase-dependent pathway.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)31684-8