Kinetic study on starch hydrolysis using a glucose/maltose sensing system

A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 mu L sample size with recycled back-flow minimises an...

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Bibliographic Details
Published in:Biotechnology techniques 1995-06, Vol.9 (6), p.445-450
Main Authors: HONG-BO QU, XIAN-EN ZHANG, WEN-WU CHUI, SHU-ZHENG ZHANG, GAO-XIANG LI, OUYANG, F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Miscellaneous
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Summary:A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 mu L sample size with recycled back-flow minimises any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00160834