Pseudorecombination and complementation between potato yellow mosaic geminivirus and tomato golden mosaic geminivirus

• Published in: Journal of general virology 1995-11, Vol.76 (11), p.2809-2815
• Main Authors: Sung, Y. K, Coutts, R. H. A
• Format: Article
• Language: English
• Subjects: Base Sequence; DNA Primers; DNA Viruses - metabolism; DNA, Viral - genetics; Geminiviridae - genetics; Geminiviridae - metabolism; Geminiviridae - physiology; Genetic Complementation Test; Molecular Sequence Data; Mutation; Nicotiana - metabolism; Nicotiana - virology; Nicotiana benthamiana; Plant Leaves - virology; Plants, Toxic; potato yellow mosaic virus; Reassortant Viruses - genetics; Reassortant Viruses - pathogenicity; Recombination, Genetic; Solanum lycopersicum - virology; Solanum tuberosum - virology; tomato golden mosaic virus; Virus Replication
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-76-11-2809

Biology Department, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, UK Pseudorecombinants made by exchanging the cloned, infectious genome components (DNAs A and B) of potato yellow mosaic geminivirus (PYMV) and the common strain (cs) of tomato golden mosaic geminivirus (csTGMV) are not infectious in their common host Nicotiana benthamiana . In an N. benthamiana leaf disc assay neither PYMV DNA A nor TGMV DNA A trans -replicated each other's DNA B component. The ability of PYMV and TGMV to mediate the systemic movement of each other's DNA A was investigated following co-inoculation of N. benthamiana with both genome components of one virus (the helper virus) and DNA A of the other virus (the dependent virus). Movement of the dependent virus DNA A in both cases illustrates interchangeability between the DNA B-encoded movement proteins of New World geminiviruses which infect solanaceous hosts. We have studied this genetic interchangeability further in separate co-agroinoculation experiments with N. benthamiana plants using TGMV DNA A to complement mutations in PYMV open reading frame (ORF) AC2, which encodes a protein that trans -activates the expression of virion sense promoters, and in PYMV ORF AC3, which specifies a protein that enhances viral DNA replication. TGMV DNA A complemented a PYMV AC2 mutant and restored its infectivity and it also complemented a PYMV AC3 mutant and restored the reduced DNA phenotype. * Author for correspondence. Fax [phone]. e-mail [e-mail] Received 5 May 1995; accepted 11 July 1995.