Effectiveness of Talc as Adsorbent for Purification and Immobilization of Plant Lipoxygenases

The present study describes the immobilization of lipoxygenase from Solanum tuberosum tuber and Lupinus albus, Cicer arietinum, and Pisum sativum seeds on a magnesium silicate based support, talc. In all cases, an increase in specific activity of many hundreds of units per milligram was obtained and...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1994-10, Vol.42 (10), p.2115-2119
Main Authors: Battu, Serge, Rabinovitch-Chable, Helene, Beneytout, Jean-L
Format: Article
Language:English
Subjects:
AGENT DE TEXTURE
Biological and medical sciences
Biotechnology
CICER ARIETINUM
ESTABILIZADORES
Fundamental and applied biological sciences. Psychology
IMMOBILISATION
Immobilization of enzymes and other molecules
Immobilization techniques
INMOVILIZACION
LIPOXIGENASA
LIPOXYGENASE
LUPINUS ALBUS
Methods. Procedures. Technologies
NUTRIENTES MINERALES
PISUM SATIVUM
PURIFICACION
PURIFICATION
SOLANUM TUBEROSUM
SUBSTANCE NUTRITIVE MINERALE
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Summary:The present study describes the immobilization of lipoxygenase from Solanum tuberosum tuber and Lupinus albus, Cicer arietinum, and Pisum sativum seeds on a magnesium silicate based support, talc. In all cases, an increase in specific activity of many hundreds of units per milligram was obtained and the use of free or immobilized enzymes was quite similar. The adsorption of plant lipoxygenases on talc was a direct, rapid, and inexpensive method to purify and stabilize these enzymes
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00046a008