Whey proteins have beneficial effects on intestinal enteroendocrine cells stimulating cell growth and increasing the production and secretion of incretin hormones

•First investigation of the effects of the 3 major whey proteins on EE cells.•BLG stimulates proliferation of intestinal EE cells and also secretion of GLP-1.•BLG’s activity appears to be lost following hydrolysis with trypsin or chymotrypsin.•ALA is a highly potent GLP-1 secretagogue & increase...

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Bibliographic Details
Published in:Food chemistry 2015-12, Vol.189, p.120-128
Main Authors: Gillespie, Anna L., Calderwood, Danielle, Hobson, Laura, Green, Brian D.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cell Proliferation - drug effects
Cell Survival - drug effects
Diabetes
Enteroendocrine Cells - drug effects
Enteroendocrine Cells - metabolism
GIP
GLP-1
Glucagon-Like Peptide 1 - secretion
Hypoglycemic Agents - pharmacology
Incretin hormones
Incretins - secretion
Intestines - cytology
Intestines - drug effects
Intestines - metabolism
Lactalbumin - analysis
Lactoglobulins - analysis
Lactoglobulins - pharmacology
Mice
pGIP/Neo STC-1
Protein
Protein Hydrolysates - pharmacology
Whey
Whey Proteins - pharmacology
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Summary:•First investigation of the effects of the 3 major whey proteins on EE cells.•BLG stimulates proliferation of intestinal EE cells and also secretion of GLP-1.•BLG’s activity appears to be lost following hydrolysis with trypsin or chymotrypsin.•ALA is a highly potent GLP-1 secretagogue & increases intracellular GLP-1 levels. Whey protein has been indicated to curb diet-induced obesity, glucose intolerance and delay the onset of type 2 diabetes mellitus. Here the effects of intact crude whey, intact individual whey proteins and beta-lactoglobulin hydrolysates on an enteroendocrine (EE) cell model were examined. STC-1 pGIP/neo cells were incubated with several concentrations of yogurt whey (YW), cheese whey (CW), beta-lactoglobulin (BLG), alpha-lactalbumin (ALA) and bovine serum albumin (BSA). The findings demonstrate that BLG stimulates EE cell proliferation, and also GLP-1 secretion (an effect which is lost following hydrolysis with chymotrypsin or trypsin). ALA is a highly potent GLP-1 secretagogue which also increases the intracellular levels of GLP-1. Conversely, whey proteins and hydrolysates had little impact on GIP secretion. This appears to be the first investigation of the effects of the three major proteins of YW and CW on EE cells. The anti-diabetic potential of whey proteins should be further investigated.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2015.02.022