Immunocytochemical localization of the plasma membrane calcium pump, calbindin-D sub(28k), and parvalbumin in Purkinje cells of avian and mammalian cerebellum

A monoclonal antibody produced against the human erythrocyte plasma membrane calcium pump (PMCA) was shown to react immunohistochemically with an epitope of the PMCA in avian and mammalian cerebellum. Dual-imaging confocal immunofluorescence microscopy of avian cerebellum showed that the PMCA antibo...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1993-01, Vol.90 (24), p.11949-11953
Main Authors: Tolosa de Talamoni, N, Smith, CA, Wasserman, R H, Beltramino, C, Fullmer, C S, Penniston, J T
Format: Article
Language:English
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Summary:A monoclonal antibody produced against the human erythrocyte plasma membrane calcium pump (PMCA) was shown to react immunohistochemically with an epitope of the PMCA in avian and mammalian cerebellum. Dual-imaging confocal immunofluorescence microscopy of avian cerebellum showed that the PMCA antibody stained the periphery of the soma whereas calbindin-D sub(28k) was located in the cytosol. PMCA heavily stained the more distal dendrites of the Purkinje cells and, within the resolution of the fluorescence procedure, colocalized with calbindin-D sub(28k). The rapid decline of intracellular free Ca super(2+) after the rapid rise time of Ca super(2+) transients is considered to be due to sequestration by Ca super(2+) buffers, uptake by intracellular stores, and Ca super(2+) extrusion mechanisms, the latter a function of PMCA now shown immunohistochemically to be a prominent feature of Purkinje cell dendrites.
ISSN:0027-8424