Anti-inflammatory Flavonoids Isolated from Passiflora foetida

• Published in: Natural product communications 2015-06, Vol.10 (6), p.929-931
• Main Authors: Nguyen, Thi Yen, To, Dao Cuong, Tran, Manh Hung, Lee, Joo Sang, Lee, Jeong Hyung, Kim, Jeong Ah, Woo, Mi Hee, Min, Byung Sun
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Cell Line; Flavonoids - chemistry; Flavonoids - isolation & purification; Flavonoids - pharmacology; Macrophages - drug effects; Macrophages - immunology; Mice; NF-kappa B - genetics; NF-kappa B - immunology; Nitric Oxide - immunology; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - immunology; Passiflora - chemistry; Plant Bark - chemistry
• ISSN: 1934-578X; 1555-9475
• DOI: 10.1177/1934578X1501000634

In this study, we evaluated the anti-inflammatory activity of the soluble ethyl acetate fraction and chemical components of the stem bark of Passiflora foetida (Passifloraceae). Ten flavonoids (1–10) were isolated by various chromatographic techniques, and their structures were determined based on spectroscopic analyses by using nuclear magnetic resonance (NMR). Luteolin (2) and chrysoeriol (3) showed the most potent inhibition of nitric oxide (NO) production in macrophage cell line, RAW264.7, with half maximal inhibitor concentration (IC50) values of 1.2 and 3.1 μM, respectively. These compounds suppressed lipopolysaccharide (LPS)-induced inducible NO synthase (iNOS) expression at the transcription level. Our research indicates that the stem bark of P. foetida has significant anti-inflammatory properties, suggesting that its flavonoids may have anti-inflammatory benefits.