Ergosterol of Cordyceps militaris Attenuates LPS Induced Inflammation in BV2 Microglia Cells

Different solvent extracts of Cordyceps militaris stroma powder were tested for cell viability and inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS) triggered BV2 microglia cells. Chemical investigation of the ethyl acetate fraction resulted in an enriched ergosterol sub-fractio...

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Bibliographic Details
Published in:Natural product communications 2015-06, Vol.10 (6), p.885-886
Main Authors: Nallathamby, Neeranjini, Guan-Serm, Lee, Vidyadaran, Sharmili, Malek, Sri Nurestri Abd, Raman, Jegadeesh, Sabaratnam, Vikineswary
Format: Article
Language:English
Subjects:
Cell Survival - drug effects
Cordyceps - chemistry
Ergosterol - pharmacology
Humans
Inflammation - drug therapy
Inflammation - immunology
Inflammation - physiopathology
Lipopolysaccharides - immunology
Microglia - drug effects
Microglia - immunology
Nitric Oxide - immunology
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Summary:Different solvent extracts of Cordyceps militaris stroma powder were tested for cell viability and inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS) triggered BV2 microglia cells. Chemical investigation of the ethyl acetate fraction resulted in an enriched ergosterol sub-fraction CE3. The BV2 cells showed no cytotoxic effects when treated with the ethyl acetate fraction and sub-fraction CE3 at concentrations of 0.1 μg/mL – 100 μg/mL compared with the control. At 10 μg/mL, the ethyl acetate fraction and sub-fraction CE3 had the highest reduction of 48.0% and 44.7% of nitric oxide production, respectively. The major compound in sub-fraction CE3 was ergosterol, identified by GCMS, and the purity was checked by HPLC. Further, the reduction of nitric oxide in LPS triggered BV2 cells was about three fold higher when compared with the control commercial ergosterol.
ISSN:1934-578X
1555-9475
DOI:10.1177/1934578X1501000623