Flow cytometry for monitoring contaminant exposure in black-crowned night-herons

The flow cytometry methods (FCM) was employed to determine cellular DNA content of black-crowned night-heron (Nycticorax nycticorax) embryos and 10-day-old chicks collected at sites differing in types of chemical contamination. The coefficient of variation of DNA content (CV) in blood collected from...

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Bibliographic Details
Published in:Archives of environmental contamination and toxicology 1994-08, Vol.27 (2), p.176-179
Main Authors: CUSTER, T. W, BICKHAM, J. W, LYNE, T. B, LEWIS, T, RUEDAS, L. A, CUSTER, C. M, MELANCON, M. J
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Animals
Applied ecology
Aves
Biological and medical sciences
Birds - genetics
Brackish
DNA - analysis
DNA - blood
DNA - drug effects
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Environmental Monitoring - methods
Environmental Pollutants - toxicity
Flow Cytometry
Freshwater
Fundamental and applied biological sciences. Psychology
Liver - chemistry
Nycticorax nycticorax
Spleen - chemistry
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Summary:The flow cytometry methods (FCM) was employed to determine cellular DNA content of black-crowned night-heron (Nycticorax nycticorax) embryos and 10-day-old chicks collected at sites differing in types of chemical contamination. The coefficient of variation of DNA content (CV) in blood collected from embryos suggested cytogenetic damage at a side in Louisiana known to be contaminated with petroleum. Blood CV from chicks suggested genetic damage at a site in Texas also known to be contaminated with petroleum. Spleen CVs in chicks were significantly lower than respective means from the reference site. The CVs of chick blood and liver and spleen negatively correlated, suggesting recovery of spleen and liver cells after exposure to a clastogenic compound. Thus, the lower CVs may also have been indicative of genetic damage. Based on the findings of this study, FCM is a potential indicator of certain environmental contaminants in black-crowned night-herons.
ISSN:0090-4341
1432-0703
DOI:10.1007/BF00214260