Octopus pallidus cytochrome P-450: characterisation and induction studies with β-naphthoflavone and Aroclor 1254

Levels of cytochrome P-450 and related enzyme activities in Octopus pallidus were investigated for the first time to assess their potential for use as biomarkers of xenobiotic exposure. Spectral analysis revealed cytochrome P-450 in the digestive gland microsomes. Enzyme assays 7-ethoxyresorufin O-d...

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Bibliographic Details
Published in:Aquatic toxicology 1995, Vol.33 (3), p.201-214
Main Authors: Cheah, D.M.Y., Wright, P.F.A., Holdway, D.A., Ahokas, J.T.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
Aroclor 1254
Biological and medical sciences
Cytochrome P-450
ECOD
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on protozoa and invertebrates
EROD
Fundamental and applied biological sciences. Psychology
Marine
Octopus pallidus
β-Naphthoflavone
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Summary:Levels of cytochrome P-450 and related enzyme activities in Octopus pallidus were investigated for the first time to assess their potential for use as biomarkers of xenobiotic exposure. Spectral analysis revealed cytochrome P-450 in the digestive gland microsomes. Enzyme assays 7-ethoxyresorufin O-deethylase and 7-ethoxycoumarin O-deethylase were optimised with respect to temperature, pH and incubation time and NADPH cytochrome c reductase assays were performed. Corn oil was demonstrated to have an inhibitory effect on octopus cytochrome P-450 system. The study with Aroclor 1254 suspended in glycerol (100 mg/kg) revealed an elevation of 7-ethoxycoumarin O-deethylase activity, which would indicate cytochrome P-450 was induced.
ISSN:0166-445X
1879-1514
DOI:10.1016/0166-445X(95)00014-U