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PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania
The members of the Anopheles maculipennis complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in...
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| Published in: | Acta parasitologica 2015-06, Vol.60 (2), p.283-289 |
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| container_end_page | 289 |
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| container_start_page | 283 |
| container_title | Acta parasitologica |
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| creator | Ivanescu, Maria-Larisa Acatrinei, Dumitru Pavel, Ionuţ Sulesco, Tatiana Miron, Liviu |
| description | The members of the
Anopheles maculipennis
complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the
Anopheles maculipennis
complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of
A. maculipennis
among which: 58
A. atroparvus
, 18
A. melanoon
, 2
A. labranchiae
, 52
A. maculipennis
and 87
A. messeae.
The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the
A. maculipennis
available in GenBank. The Species
A. labranchiae
is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the
A. labranchiae
sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country. |
| doi_str_mv | 10.1515/ap-2015-0040 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1698958013</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4312243481</sourcerecordid><originalsourceid>FETCH-LOGICAL-c408t-91fee2066af096f34fd61962012ccee5863d05e3cdb38fb51e77e491db9b254e3</originalsourceid><addsrcrecordid>eNqFkd1rFTEQxRdR7Ie--SwBX1owmo9NbtK3clu1UFSKPodsdtKbspusya5t_3tzuVVEhD7NMPObk0NO07yi5B0VVLy3E2aECkxIS540-1RpiakS9GntGSeYKUb3moNSbiohlVLPmz0mGeFar_abu6_rKxR6iHPwwdk5pIiSRz78BFQmcAEK8jmNaN4AOo1p2sBQR6N1yxAmiDEU5NI4DXCHjs7CNEO2J2hdly70Fo5RiOhzyvMGn9tSlxFdpdHGYF80z7wdCrx8qIfN9w_n39af8OWXjxfr00vsWqJmrKkHYERK64mWnre-l1RX-5Q5ByCU5D0RwF3fceU7QWG1glbTvtMdEy3ww-Zopzvl9GOBMpsxFAfDYCOkpRgqtdJCEcor-uYf9CYtOVZ3hiopGJNak0q93VEup1IyeDPlMNp8bygx20SMncw2EbNNpOKvH0SXboT-D_w7ggqc7IBbO9QP6uE6L_e1-evx_-hKwpjaWsa741J14_VjV_wXIlmn5Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1865226990</pqid></control><display><type>article</type><title>PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania</title><source>Springer Link</source><creator>Ivanescu, Maria-Larisa ; Acatrinei, Dumitru ; Pavel, Ionuţ ; Sulesco, Tatiana ; Miron, Liviu</creator><creatorcontrib>Ivanescu, Maria-Larisa ; Acatrinei, Dumitru ; Pavel, Ionuţ ; Sulesco, Tatiana ; Miron, Liviu</creatorcontrib><description>The members of the
Anopheles maculipennis
complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the
Anopheles maculipennis
complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of
A. maculipennis
among which: 58
A. atroparvus
, 18
A. melanoon
, 2
A. labranchiae
, 52
A. maculipennis
and 87
A. messeae.
The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the
A. maculipennis
available in GenBank. The Species
A. labranchiae
is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the
A. labranchiae
sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1515/ap-2015-0040</identifier><identifier>PMID: 26203997</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Systematics/Taxonomy/Biogeography ; Animals ; Anopheles - classification ; Anopheles - genetics ; Anopheles maculipennis complex ; Base Sequence ; Biomedical and Life Sciences ; Biomedicine ; Cluster Analysis ; DNA, Ribosomal Spacer - genetics ; Ecology ; Europe ; Global warming ; Humidity ; Insect Vectors ; Malaria ; Medical Microbiology ; Microbiology ; Molecular Sequence Data ; Mosquitoes ; Parasitology ; Phylogeny ; Polymerase Chain Reaction ; Ribosomal DNA ; Romania ; Sequence Analysis, DNA ; Veterinary medicine ; Zoology</subject><ispartof>Acta parasitologica, 2015-06, Vol.60 (2), p.283-289</ispartof><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2015</rights><rights>Copyright Walter de Gruyter GmbH 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-91fee2066af096f34fd61962012ccee5863d05e3cdb38fb51e77e491db9b254e3</citedby><cites>FETCH-LOGICAL-c408t-91fee2066af096f34fd61962012ccee5863d05e3cdb38fb51e77e491db9b254e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26203997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ivanescu, Maria-Larisa</creatorcontrib><creatorcontrib>Acatrinei, Dumitru</creatorcontrib><creatorcontrib>Pavel, Ionuţ</creatorcontrib><creatorcontrib>Sulesco, Tatiana</creatorcontrib><creatorcontrib>Miron, Liviu</creatorcontrib><title>PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>The members of the
Anopheles maculipennis
complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the
Anopheles maculipennis
complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of
A. maculipennis
among which: 58
A. atroparvus
, 18
A. melanoon
, 2
A. labranchiae
, 52
A. maculipennis
and 87
A. messeae.
The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the
A. maculipennis
available in GenBank. The Species
A. labranchiae
is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the
A. labranchiae
sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country.</description><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Animals</subject><subject>Anopheles - classification</subject><subject>Anopheles - genetics</subject><subject>Anopheles maculipennis complex</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cluster Analysis</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Ecology</subject><subject>Europe</subject><subject>Global warming</subject><subject>Humidity</subject><subject>Insect Vectors</subject><subject>Malaria</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mosquitoes</subject><subject>Parasitology</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Ribosomal DNA</subject><subject>Romania</subject><subject>Sequence Analysis, DNA</subject><subject>Veterinary medicine</subject><subject>Zoology</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFkd1rFTEQxRdR7Ie--SwBX1owmo9NbtK3clu1UFSKPodsdtKbspusya5t_3tzuVVEhD7NMPObk0NO07yi5B0VVLy3E2aECkxIS540-1RpiakS9GntGSeYKUb3moNSbiohlVLPmz0mGeFar_abu6_rKxR6iHPwwdk5pIiSRz78BFQmcAEK8jmNaN4AOo1p2sBQR6N1yxAmiDEU5NI4DXCHjs7CNEO2J2hdly70Fo5RiOhzyvMGn9tSlxFdpdHGYF80z7wdCrx8qIfN9w_n39af8OWXjxfr00vsWqJmrKkHYERK64mWnre-l1RX-5Q5ByCU5D0RwF3fceU7QWG1glbTvtMdEy3ww-Zopzvl9GOBMpsxFAfDYCOkpRgqtdJCEcor-uYf9CYtOVZ3hiopGJNak0q93VEup1IyeDPlMNp8bygx20SMncw2EbNNpOKvH0SXboT-D_w7ggqc7IBbO9QP6uE6L_e1-evx_-hKwpjaWsa741J14_VjV_wXIlmn5Q</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Ivanescu, Maria-Larisa</creator><creator>Acatrinei, Dumitru</creator><creator>Pavel, Ionuţ</creator><creator>Sulesco, Tatiana</creator><creator>Miron, Liviu</creator><general>Springer International Publishing</general><general>De Gruyter Open</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania</title><author>Ivanescu, Maria-Larisa ; Acatrinei, Dumitru ; Pavel, Ionuţ ; Sulesco, Tatiana ; Miron, Liviu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-91fee2066af096f34fd61962012ccee5863d05e3cdb38fb51e77e491db9b254e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Animals</topic><topic>Anopheles - classification</topic><topic>Anopheles - genetics</topic><topic>Anopheles maculipennis complex</topic><topic>Base Sequence</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cluster Analysis</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Ecology</topic><topic>Europe</topic><topic>Global warming</topic><topic>Humidity</topic><topic>Insect Vectors</topic><topic>Malaria</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mosquitoes</topic><topic>Parasitology</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Ribosomal DNA</topic><topic>Romania</topic><topic>Sequence Analysis, DNA</topic><topic>Veterinary medicine</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ivanescu, Maria-Larisa</creatorcontrib><creatorcontrib>Acatrinei, Dumitru</creatorcontrib><creatorcontrib>Pavel, Ionuţ</creatorcontrib><creatorcontrib>Sulesco, Tatiana</creatorcontrib><creatorcontrib>Miron, Liviu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest - 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Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ivanescu, Maria-Larisa</au><au>Acatrinei, Dumitru</au><au>Pavel, Ionuţ</au><au>Sulesco, Tatiana</au><au>Miron, Liviu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>60</volume><issue>2</issue><spage>283</spage><epage>289</epage><pages>283-289</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>The members of the
Anopheles maculipennis
complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the
Anopheles maculipennis
complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of
A. maculipennis
among which: 58
A. atroparvus
, 18
A. melanoon
, 2
A. labranchiae
, 52
A. maculipennis
and 87
A. messeae.
The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the
A. maculipennis
available in GenBank. The Species
A. labranchiae
is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the
A. labranchiae
sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>26203997</pmid><doi>10.1515/ap-2015-0040</doi><tpages>7</tpages></addata></record> |
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| ispartof | Acta parasitologica, 2015-06, Vol.60 (2), p.283-289 |
| issn | 1230-2821 1896-1851 |
| language | eng |
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| subjects | Animal Systematics/Taxonomy/Biogeography Animals Anopheles - classification Anopheles - genetics Anopheles maculipennis complex Base Sequence Biomedical and Life Sciences Biomedicine Cluster Analysis DNA, Ribosomal Spacer - genetics Ecology Europe Global warming Humidity Insect Vectors Malaria Medical Microbiology Microbiology Molecular Sequence Data Mosquitoes Parasitology Phylogeny Polymerase Chain Reaction Ribosomal DNA Romania Sequence Analysis, DNA Veterinary medicine Zoology |
| title | PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania |
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