On-line determination of pigment composition and biomass in cultures of microalgae

Rhodomonas sp. was grown in a photo-bioreactor equipped with a measuring cell in a spectrophotometer as part of an external flow loop. The apparent absorbance from 400 to 800 nm of the cell suspension was recorded at predetermined intervals and stored in a computer. From the spectra, the biomass and...

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Bibliographic Details
Published in:Biotechnology techniques 1995, Vol.9 (1), p.49-54
Main Authors: Eriksen, N.T, Iverson, J.J.L
Format: Article
Language:English
Subjects:
algae and seaweeds
Algal culture (microalgae)
Biological and medical sciences
biomass
bioreactors
Biotechnology
Brackish
cell culture
chlorophyll
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
monitoring
phycoerythrin
pigments
rhodomanas
Rhodomonas
spectroscopy
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Summary:Rhodomonas sp. was grown in a photo-bioreactor equipped with a measuring cell in a spectrophotometer as part of an external flow loop. The apparent absorbance from 400 to 800 nm of the cell suspension was recorded at predetermined intervals and stored in a computer. From the spectra, the biomass and the concentrations of the two pigments chlorophyll a and phycoerythrin were determined in nitrogen-limited batch cultures.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00153000