In house validation from direct determination of 5-hydroxymethyl-2-furfural (HMF) in Brazilian corn and cane syrups samples by HPLC–UV

• Published in: Food chemistry 2016-01, Vol.190, p.481-486
• Main Authors: de Andrade, Jucimara K., Komatsu, Emy, Perreault, Hélène, Torres, Yohandra R., da Rosa, Marcos R., Felsner, Maria L.
• Format: Article
• Language: English
• Subjects: 5-Hydroxymethyl-2-furfural (HMF); Brazil; Cane syrups; Carbohydrates - analysis; Chromatography, High Pressure Liquid - methods; Corn syrups; Furaldehyde - analogs & derivatives; Furaldehyde - analysis; HPLC–UV; In house validation; Spectrophotometry, Ultraviolet; Zea mays - chemistry
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2015.05.131

•In house validation of HMF direct determination in syrups by HPLC–UV is investigated.•HPLC method showed excellent performance and low limits (LOD and LOQ).•Developed method is fast and direct and can be applied in food laboratories.•Very high HMF contents in Brazilian cane and corn syrups were found.•High HMF levels in Brazilian syrups arouses concern about food safety these foods. In this work the development and in house validation of the HMF direct determination in corn and cane syrups by HPLC–UV was carried out for the first time. The separation was done with isocratic elution of a mobile phase comprising water (with 0.5% formic acid) and acetonitrile (90:10, v/v) on Phenomenex C18 column (5.0μm, 4.6×150mm), at 30°C, flow rate of 0.8mLmin−1 and detection at 285nm. The validated method showed excellent performance with low limits (LOD and LOQ of 0.09 and 0.26mgL−1, respectively), good accuracy (recovery rates between 100% and 104%) and precision (RSD’s for repeatability and intermediate precision between 0.57% and 6.43%). Good selectivity and linearity were also observed. HMF contents in both foods were very high (406.6–2121.3mgkg−1 for corn syrup and 109.2–893.1mgkg−1 for cane syrup), which arouses concern about food safety of these products.