In vivo immunological activity of carboxymethylated-sulfated (1→3)-β-d-glucan from sclerotium of Poria cocos

• Published in: International journal of biological macromolecules 2015-08, Vol.79, p.511-517
• Main Authors: Wang, Haili, Mukerabigwi, Jean Felix, Zhang, Yuannian, Han, Lin, Jiayinaguli, Telieke, Wang, Qing, Liu, Lina, Cao, Yu, Sun, Renqiang, Huang, Xueying
• Format: Article
• Language: English
• Subjects: Animals; Antibodies - blood; Antibody Formation - drug effects; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antioxidants - chemistry; Antioxidants - pharmacology; Carbohydrate Conformation; Carboxymethylated-sulfated (1→3)-β-d-glucan; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - pharmacology; Glucans - chemistry; Glucans - pharmacology; Hemolysin Proteins - immunology; Hep G2 Cells; Humans; Immunologic Factors - isolation & purification; Immunologic Factors - pharmacology; Immunological activity; In vivo; Liver - drug effects; Liver - metabolism; Male; Medicine, Chinese Traditional; Mice, Inbred BALB C; Poria - chemistry; Spleen - drug effects; Xenograft Model Antitumor Assays
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2015.05.020

β-Glucans are one of the polysaccharides known as biological response modifiers extracted from the sclerotium of Poria cocos which has been used for several decades as Traditional Chinese Medicine. Due to its ability to activate immune system, it can be applied in chemotherapy after being chemically modified. In this study, sulfated (1→3)-β-d-glucan (S-P), carboxymethyl (1→3)-β-d-glucan (CMP), and carboxymethylated-sulfated (1→3)-β-d-glucan (S-CMP), which are (1→3)-β-d-glucan derivatives were synthesized. The current study was aimed to investigate in vivo potential immunological activity of S-CMP in mice. In addition, mice were separately treated with S-P, CMP and S-CMP to evaluate the relationship between single and multiple functional groups. Interestingly, S-CMP exhibited the best in vivo immunological activities and the highest inhibition rate against the implanted HepG2 tumor in BALB/c mice, with significant increase in serum hemolysin antibody titer, spleen antibody production as well as delayed type hypersensitivity compared with S-P and CMP. Furthermore, it was assumed that simultaneous introduction of carboxymethyl and sulfate groups also had great potential effect on antioxidant activity, as substantial decrease in malondialdehyde (MDA) content was remarked. Therefore, it may suggest that S-CMP has better immunological and anti-tumor effects on mice in vivo.