Glia-dependent neurotoxicity and neuroprotection in mesencephalic cultures

Dopaminergic neurotoxicities of 6-hydroxydopamine (6-OHDA) and the lipopolysaccharide (LPS) were compared in rat mesencephalic cultures plated on poly- l-lysine or on glial monolayers. In the neuron-enriched cultures plated on polylysine, 6-OHDA killed 89% of the tyrosine hydroxylase (TH)-immunoposi...

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Bibliographic Details
Published in:Brain research 1995-12, Vol.704 (1), p.112-116
Main Authors: Bronstein, D.M., Perez-Otano, I., Sun, V., Mullis Sawin, S.B., Chan, J., Wu, G.-C., Hudson, P.M., Kong, L.-Y., Hong, J.-S., McMillian, M.K.
Format: Article
Language:English
Subjects:
6-hydroxydopamine
Animals
Astrocyte
Biological and medical sciences
Cell Count
Cells, Cultured
Dopamine
Fundamental and applied biological sciences. Psychology
Isolated neuron and nerve. Neuroglia
Lipopolysaccharide
Lipopolysaccharides - toxicity
Mesencephalon - cytology
Mesencephalon - drug effects
Microglia
Neuroglia - drug effects
Neurons - drug effects
Neurons - enzymology
Neuroprotective Agents - metabolism
Neurotoxins - toxicity
Oxidopamine - toxicity
Parkinson's disease
Polylysine
Rats
Substantia nigra
Tyrosine 3-Monooxygenase - analysis
Tyrosine hydroxylase
Vertebrates: nervous system and sense organs
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Summary:Dopaminergic neurotoxicities of 6-hydroxydopamine (6-OHDA) and the lipopolysaccharide (LPS) were compared in rat mesencephalic cultures plated on poly- l-lysine or on glial monolayers. In the neuron-enriched cultures plated on polylysine, 6-OHDA killed 89% of the tyrosine hydroxylase (TH)-immunopositive neurons, but LPS was not neurotoxic. Conversely, in mixed neuron/glial cultures, 6-OHDA killed only 27% of the TH-immunopositive neurons while LPS killed 70%. The mixed neuronal/glial mesencephalic culture offers a better in vitro model for studying possible mechanisms involved in Parkinson's disease.
ISSN:0006-8993
1872-6240
DOI:10.1016/0006-8993(95)01189-7