Pomphorhynchus laevis (Acanthocephala) from the Sava River basin: New insights into strain formation, mtDNA-like sequences and dynamics of infection

Abstract Here we report the genetic variability and presence of mtDNA-like sequences of Pomphorhynchus laevis from the chub, Squalius cephalus , caught at the sampling sites along the Sava River and its tributary the Sutla River in Croatia. Sequences of the cytochrome c oxidase subunit 1 (COI) gene...

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Published in:Parasitology international 2015-10, Vol.64 (5), p.243-250
Main Authors: Vardić Smrzlić, Irena, Valić, Damir, Kapetanović, Damir, Filipović Marijić, Vlatka, Gjurčević, Emil, Teskeredžić, Emin
Format: Article
Language:English
Subjects:
Acanthocephala - classification
Acanthocephala - genetics
Acanthocephala - isolation & purification
Animals
Base Sequence
Croatia - epidemiology
Cyprinidae - parasitology
DNA, Helminth - chemistry
DNA, Helminth - genetics
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
Electron Transport Complex IV - genetics
Female
Fish Diseases - epidemiology
Fish Diseases - parasitology
Gastroenterology and Hepatology
Genetic Variation
Haplotypes
Helminthiasis - epidemiology
Helminthiasis - parasitology
Infectious Disease
Male
Molecular Sequence Data
Phylogeny
Rivers
Sequence Analysis, DNA - veterinary
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Summary:Abstract Here we report the genetic variability and presence of mtDNA-like sequences of Pomphorhynchus laevis from the chub, Squalius cephalus , caught at the sampling sites along the Sava River and its tributary the Sutla River in Croatia. Sequences of the cytochrome c oxidase subunit 1 (COI) gene of the recovered P. laevis specimens were used for haplotype network construction and phylogenetic analysis. These analyses showed that some specimens contained mitochondrial-like sequences, and they uncovered the existence of a Sava River basin strain different from known strains of P. laevis . This is the first time that P. laevis has been shown to contain mtDNA-like sequences, suggesting the need to exercise caution during COI analyses of P. laevis using universal primers. Highly conserved sequences of two nuclear markers, the ITS region and 18S rRNA, were not helpful for understanding genetic variability or differentiating strains. Furthermore, analysis of the dynamics of P. laevis infections in S. cephalus from the Sava and Sutla Rivers showed decreased prevalence and abundance at sites with inferior water quality, positive association of parasite abundance with fish size, and no clear association of parasite abundance with fish condition index or sex.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2015.02.004