Early Detection of Systemic Rust Infections of Dyers Woad (Isatis tinctoria) Using the Polymerase Chain Reaction

Rust-specific polymerase chain reaction (PCR) primers selectively amplified ribosomal DNA of a rust fungus from infected dyers woad. PCR enabled DNA of the fungus to be detected in symptomatic plants as well as in asymptomatic parts of diseased plants. The use of PCR enabled early detection of rust...

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Bibliographic Details
Published in:Weed science 1995, Vol.43 (3), p.467-472
Main Authors: Kropp, Bradley R., Albee, Steve, Flint, Karen M., Zambino, Paul, Szabo, Les, Thomson, Sherman V.
Format: Article
Language:English
Subjects:
adn
auxiliaire de lutte biologique
biological control
biological control organisms
control biologico
desherbage
dna
Early detection
escarda
Fungi
herbicidas
herbicide
herbicides
Infections
Isatis tinctoria
Leaves
lutte biologique
microbial pesticides
organismos para control biologico
pathogenese
pathogenesis
patogenesis
pcr
pesticide microbien
plaguicidas microbianos
Plant products
Plants
Polymerase chain reaction
puccinia
Ribosomal DNA
ribosomas
ribosome
ribosomes
rouille
roya
rusts
Special Topics
Symptoms
Teliospores
weed control
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Description
Summary:Rust-specific polymerase chain reaction (PCR) primers selectively amplified ribosomal DNA of a rust fungus from infected dyers woad. PCR enabled DNA of the fungus to be detected in symptomatic plants as well as in asymptomatic parts of diseased plants. The use of PCR enabled early detection of rust infections in dyers woad plants during their first season when they are often asymptomatic. Dried plant samples stored at room temperature for several months worked as well as lyophilized material for DNA extraction prior to PCR. The PCR detection method should greatly facilitate further studies on the biology and inoculation of this and other systemic rusts that have potential for use in biocontrol of weeds.
ISSN:0043-1745
1550-2759
DOI:10.1017/S0043174500081480