Selectable cassettes for simplified construction of yeast gene disruption vectors

Cassettes based on a hisG-URA3-hisG insert have been modified by the addition of a Km R-encoding gene and flanking polylinker sites, greatly simplifying construction of gene disruption vectors in Escherichia coli. After gene disruption in yeast, URA3 can then be excised by recombination between the...

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Bibliographic Details
Published in:Gene 1996-02, Vol.169 (1), p.111-113
Main Authors: Earley, Marie C., Crouse, Gray F.
Format: Article
Language:English
Subjects:
Cloning, Molecular - methods
Escherichia coli
gene targeting
Genetic Vectors
Kanamycin Resistance
Molecular Sequence Data
Mutagenesis, Insertional - methods
plasmid
Recombination
Recombination, Genetic
Saccharomyces cerevisiae - genetics
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Summary:Cassettes based on a hisG-URA3-hisG insert have been modified by the addition of a Km R-encoding gene and flanking polylinker sites, greatly simplifying construction of gene disruption vectors in Escherichia coli. After gene disruption in yeast, URA3 can then be excised by recombination between the hisG repeats flanking the gene, permitting reuse of the URA3 marker
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00805-5