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Capillary electrophoresis for the characterization of humic substances
The potential of high performance capillary electrophoresis (HPCE), especially in the free solution mode (FSCE), is demonstrated for the analysis/characterization of environmental humic substances (HUS). The very high efficiency of HPCE separations allows the production of electropherograms that are...
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Published in: | Water research (Oxford) 1995, Vol.29 (9), p.2149-2159 |
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container_title | Water research (Oxford) |
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creator | Garrison, A.W. Schmitt, P. Kettrup, A. |
description | The potential of high performance capillary electrophoresis (HPCE), especially in the free solution mode (FSCE), is demonstrated for the analysis/characterization of environmental humic substances (HUS). The very high efficiency of HPCE separations allows the production of electropherograms that are more characteristic for HUS than are liquid chromatograms; in addition, HUS can be analyzed by FSCE in their naturally occurring anionic forms. Fulvic and humic acid fractions of HUS extracted from soil and water by procedures of the International Humic Substances Society were analyzed using an uncoated silica column and acetate, borate or phosphate buffer systems to produce electropherograms of the anionic species. Soil fulvic acids exhibit a consistent and characteristic set of sharp peaks, extending from a humic “hump”; migration times range between 5 and 12 min depending on soil source, buffer and pH. Humic acids give only the “hump,” which sometimes has shoulders but little definition. Soil and river humic acids produce only slightly different electropherograms at pH 8.30 in a borate buffer system, and each shows a remarkable peak-sharpening as the pH is increased to just 8.55. The electropherograms of “young” and “old” groundwater fulvic acids show different fingerprints. |
doi_str_mv | 10.1016/0043-1354(95)00031-F |
format | article |
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The very high efficiency of HPCE separations allows the production of electropherograms that are more characteristic for HUS than are liquid chromatograms; in addition, HUS can be analyzed by FSCE in their naturally occurring anionic forms. Fulvic and humic acid fractions of HUS extracted from soil and water by procedures of the International Humic Substances Society were analyzed using an uncoated silica column and acetate, borate or phosphate buffer systems to produce electropherograms of the anionic species. Soil fulvic acids exhibit a consistent and characteristic set of sharp peaks, extending from a humic “hump”; migration times range between 5 and 12 min depending on soil source, buffer and pH. Humic acids give only the “hump,” which sometimes has shoulders but little definition. Soil and river humic acids produce only slightly different electropherograms at pH 8.30 in a borate buffer system, and each shows a remarkable peak-sharpening as the pH is increased to just 8.55. The electropherograms of “young” and “old” groundwater fulvic acids show different fingerprints.</description><subject>Analytical chemistry</subject><subject>capillary electrophoresis</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>free solution mode</subject><subject>fulvic acid</subject><subject>humic acid</subject><subject>humic substances</subject><subject>Other chromatographic methods</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqF0E1LwzAYwPEgCs7pN_DQg4geqnlr01wEGVaFgRc9hzR9QiPdUpNW0E9v5saOesrl_zxJfgidE3xDMClvMeYsJ6zgV7K4xhgzktcHaEYqIXPKeXWIZvvkGJ3E-J4iSpmcoXqhB9f3Onxl0IMZgx86HyC6mFkfsrGDzHQ6aDNCcN96dH6deZt108qZLE5NHPXaQDxFR1b3Ec525xy91Q-vi6d8-fL4vLhf5oYzMuZCMlK2IKmtKkpLKVtTQmmF5dSWgsuGNVRy0pY6PVC20Oi2Moy3gCWraCPYHF1u9w7Bf0wQR7Vy0UD6wBr8FBURGAvB5P8hK4sCFzSFfBua4GMMYNUQ3Cp5KILVRldt6NSGTslC_eqqOo1d7PbraHRvQ2JwcT_LCpF8ScruthkklE8HQUXjIIm1LiRt1Xr39z0_TXCNlw</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Garrison, A.W.</creator><creator>Schmitt, P.</creator><creator>Kettrup, A.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>1995</creationdate><title>Capillary electrophoresis for the characterization of humic substances</title><author>Garrison, A.W. ; Schmitt, P. ; Kettrup, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-79316de92f8822699dc6e6f7f42f6749b3b2941d6a0009debad8c34de09382b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Analytical chemistry</topic><topic>capillary electrophoresis</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Exact sciences and technology</topic><topic>free solution mode</topic><topic>fulvic acid</topic><topic>humic acid</topic><topic>humic substances</topic><topic>Other chromatographic methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garrison, A.W.</creatorcontrib><creatorcontrib>Schmitt, P.</creatorcontrib><creatorcontrib>Kettrup, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garrison, A.W.</au><au>Schmitt, P.</au><au>Kettrup, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capillary electrophoresis for the characterization of humic substances</atitle><jtitle>Water research (Oxford)</jtitle><date>1995</date><risdate>1995</risdate><volume>29</volume><issue>9</issue><spage>2149</spage><epage>2159</epage><pages>2149-2159</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>The potential of high performance capillary electrophoresis (HPCE), especially in the free solution mode (FSCE), is demonstrated for the analysis/characterization of environmental humic substances (HUS). The very high efficiency of HPCE separations allows the production of electropherograms that are more characteristic for HUS than are liquid chromatograms; in addition, HUS can be analyzed by FSCE in their naturally occurring anionic forms. Fulvic and humic acid fractions of HUS extracted from soil and water by procedures of the International Humic Substances Society were analyzed using an uncoated silica column and acetate, borate or phosphate buffer systems to produce electropherograms of the anionic species. Soil fulvic acids exhibit a consistent and characteristic set of sharp peaks, extending from a humic “hump”; migration times range between 5 and 12 min depending on soil source, buffer and pH. Humic acids give only the “hump,” which sometimes has shoulders but little definition. Soil and river humic acids produce only slightly different electropherograms at pH 8.30 in a borate buffer system, and each shows a remarkable peak-sharpening as the pH is increased to just 8.55. The electropherograms of “young” and “old” groundwater fulvic acids show different fingerprints.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/0043-1354(95)00031-F</doi><tpages>11</tpages></addata></record> |
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source | Elsevier:Jisc Collections:Elsevier Read and Publish Agreement 2022-2024:Freedom Collection (Reading list) |
subjects | Analytical chemistry capillary electrophoresis Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology free solution mode fulvic acid humic acid humic substances Other chromatographic methods |
title | Capillary electrophoresis for the characterization of humic substances |
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